秦岭地区北方山溪鲵的遗传多样性和种群分化

Genetic diversity and population subdivison of Batrachuperus tibetanus in Qinling Mountains

用分子系统学方法探究秦岭地区北山溪鲵的遗传多样性和种群分化。扩增测定了秦岭地区北方山溪鲵8个种群66个个体的线粒体DNA Cytb基因754bp序列,采用生物软件进行碱基组成、序列变异和遗传多样性分析。构建了单倍型的MP,ML和BI系统进化树和单倍型网络图。秦岭地区的北方山溪鲵可以划分为两个大的分支——东部进化枝和西部进化枝,东、西两个进化枝在系统进化树和网络图上都可以明显的分开;东部的种群表现出较高的单倍型频率,太白山北坡汤峪的种群表现出有较高的核苷酸多样性,并和东西两个进化枝都共有单倍型。推测秦岭地区北方山溪鲵的东西两个进化枝可能经历了隔离分化,汤峪种群是由东西两个类群扩散形成。秦岭地区北方山溪鲵受地质历史事件和气候变化影响产生了种群间的隔离分化和扩散过程。

To study the genetic diversity and population subdivision of Batrachuperus tibetanus from Qinling Mountains. The 754bp sequence of mtDNA Cytb gene of 66 individuals in the 8 populations were amplified andmeasured. Base composition, sequence variation and genetic diversity analysis were generated using biology soft- ware, the phylogenetic trees are constructed with maximum-parsimony （MP）, maximum-likelihood （ML） and Bayesian inference （BI）methods, and Haplotype network are constructed. The populations of Batrachuperus tibeta-nus in Qinling Mountains can be divided into two large clades（ East clade and West clade）, the east and west clade in Qinling Mountains are clearly separated in both the shows higher haplotype frequencies, Tangyu Population phylogenetic tree and Haplotype network. The east group shows a higher nucleotide diversity and is formed by diffu- sion of east and west groups. The geological event and the climate changes population subdivision and genetic diversity of Batrachuperus tibetanus in during the Quaternary Ice Age, bring the Qinling Mountains.