蒽酮-硫酸比色法测定盘龙七中多糖的含量

Determination of polysaccharides in Rhizoma Bergeniae Scopulosae by Anthrone-sulfuric Acid colorimetric method

建立测定盘龙七中多糖含量的方法。以无水葡萄糖为对照品，采用蒽酮一硫酸比色法，在584nm处测定盘龙七中多糖的含量。多糖在0．02—0．10mg／mL范围内呈良好的线性关系，线性回归方程为），=6．7950x＋0．0342，7=0．9994（n=5），平均回收率为101．06％，RSD=1．31％，（n=6）。结论是所用方法快速准确、重现性好，可用于盘龙七中多糖的含量测定。

To establish a method to determine the content of polysaccharides in Rhizoma Bergeniae Scopulosae. De- termination of polysaccharides in Rhizoma Bergeniae Scopulosae were tested at 584 nm by Anthrone-sulfuric Acid colorimetric method in which anhydrous Dextrose was used as standard reference. The linear range of polysaccha- rides was 0. 02 -0. 10 mg/mL, the linear equation was y =6. 795 0x ＋0. 034 2, the relevance modulus T =0. 999 4（n =5）. The average recovery was 101.06% and RSD = 1.31% （rt =6）. Conclusion： this method is rapid, accu- rate and reproducible. It can be used to determine Rhizoma Bergeniae Scopulosae.