射频磁控溅射法制备钛基HA涂层的结构与生物活性

Structure and biological activity of radio-frequency magnetron sputtering prepared hydroxyapatite coating on titanium substrate

采用射频磁控溅射法在医用钛表面制备羟基磷灰石（HA）涂层，研究HA涂层的形貌、物相、力学性能、细胞相容性和在机体内的组织相容性，分析其在骨修复中应用的可能性。结果表明：射频磁控溅射法制备的钛基HA生物涂层呈粗糙岛屿状结构，HA平均粒径为（40？2） nm、厚度为1.0~1.6μm的涂层力学性能最好，其纳米硬度高于11 GPa，弹性模量大于136 GPa；HA涂层可促进成骨细胞增殖，成骨细胞粘附于HA涂层表面并形成伪足铺展生长；植入实验动物体内4周后材料表面被结缔组织覆盖，血管形成；植入12周后，骨小梁形成，其内部可见破骨细胞；植入12周后与植入前相比，涂层的结合强度未发生显著变化。说明该 HA涂层具有较高的成骨活性和稳定性，在骨修复方面具有良好的应用前景。

Hydroxyapatite （HA） coatings on medical titanium substrate were prepared by radio-frequency（RF） magnetron sputtering method. And the morphology, phase, mechanical properties, biocompatibility and histocompatibility of the coatings in body were researched to analyze the possibility of applying in bone repair. The results show that：HA coatings on medical titanium substrate prepared by RF magnetron sputtering show rough island-like structure; average particle diameter of HA is （40±2） nm; coating with thickness of 1.0~1.6 μm shows the best mechanical properties with nanohardness beyond 11 GPa and elastic modulus beyond 136 GPa. HA coating can promote the proliferation of osteoblasts which adhered to HA coating surface, and formed pseudopodia to spread out growth. After implanted in experimental animals’ body for 4 week, material surface is covered by connective tissue and blood vessel form;and after 12 week, trabecular bone forms and osteoclasts are visible inside. The comparison between Pre-implantation and the implantation for 12 week shows that the bonding strength of coating has no significant change which indicates high osteogenic activity and stability of HA coatings. Consequently the HA coatings has a good applied prospect.