微波消解-塞曼石墨炉原子吸收光谱法检测贝类中的铬

Determination of Chromium in Shellfish Using Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometer with Zeeman Correction

建立了分析贝类样品中铬含量的微波消解一塞曼石墨炉原子吸收光谱法，并对样品前处理方法和检测条件进行探讨．实验结果表明，利用微波消解进行前处理的样品溶样均匀，样品消解完全．石墨炉铬工作曲线浓度范围设定在0．00～15．00μg·L^-1之间时，铬浓度与其吸光值呈良好的曲线关系，得出的新合理一元二次方程相关系数r=1．0000．同时对3种贝类样品进行平行测定和加标回收率测定，平行性好，加标回收率保持在82％-95％．并利用国家标准物质海带和贻贝成份分析标准物质进行质量控制，得到的测定值在标准证书的允许误差范围内，相对标准偏差分别为2．93％和2．54％．表明该方法具有操作简便、灵敏度高、准确可靠等优点，适合贝类等水产品中铬含量的检测．

In this paper, the method for determining chromium in shellfish is studied using Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometry （GF-AAS） with Zeeman correction. The results of analysis demonstrate that by Microwave Digestion, the samples are digested evenly and completely. When chromium working curve is linear within the range of 0.00-15.00μg·L^-1, it is found that chromium concentration can be described by a smooth curve with the correlation coefficient r=1.0000. Based on this working curve, the results of Sinonovacula constricta can be determined. Comparing Tegillarca granosa with Mussel in their recovery rates, it is found that the latter can reach as high as 82%-95%. Moreover, the accuracy and precision of this method are also tested with the national standard substance （Laminaria japonica Aresch and Mussel）. It shows that the errors of the results fall within the permissible range. The relative standard deviations were 2.93% and 2.54%. To conclude, with high sensibility, high quality and simple procedure, the Microwave Digestion and Graphite Furnace Atomic Absorption Spectrometry （GF-AAS） equipped with Zeeman correction, is suitable for determination of chromium in shellfish samples.