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急性跑台运动对骨骼肌氧化应激及Fas/FasL、Bcl-2/Bax基因表达的影响 被引量:5

Effects of acute treadmill exercise on oxidative stress and apoptosis regulating gene expressions in skeletal muscle
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摘要 目的:通过检测急性跑台运动中骨骼肌氧化应激情况以及Fas/FasL、Bcl-2/Bax等凋亡调控基因表达的动态变化,探究急性跑台运动诱导骨骼肌细胞凋亡的分子机制。方法:以小鼠急性递增负荷跑台运动为实验模型,连续观察与分别测定安静组(Con)、急性跑台运动45min组(E45)、90min组(E90)、120min组(E120)和150min组(E150)的腓肠肌丙二醛(MDA)、过氧化氢(H2O2)、一氧化氮(NO)含量和超氧化物歧化酶(SOD)活性变化以及Fas/FasL、Bcl-2/Bax基因表达水平。结果:①骨骼肌MDA和H2O2含量在急性跑台运动第45分钟时显著升高(P<0.05),维持一段时间后又稳步下降,直到运动结束;骨骼肌SOD活性却呈现与之基本相反的变化态势;骨骼肌NO含量在急性跑台运动第45分钟时显著升高(P<0.01),并始终维持较高水平,直到运动结束。②骨骼肌Fas/FasL mRNA表达水平在急性跑台运动第90—120分钟时显著上升(P<0.01),随后有稳步下降态势;骨骼肌Bcl-2 mRNA表达水平在急性跑台运动第90—120分钟时显著降低(P<0.05),随后有稳步上升态势;骨骼肌Bax mRNA表达水平在急性跑台运动第45分钟时显著上升(P<0.05),在第90分钟时达到峰值(P<0.01),维持到第120分钟(P<0.05)后呈现下降态势;Bcl-2/Bax比率在急性跑台运动第45分钟时显著降低(P<0.05),在90min时达最低水平(P<0.01),维持到第120分钟(P<0.05)后呈现上升态势。结论:急性跑台运动可使骨骼肌MDA、H2O2、NO含量显著上升、SOD活性下降,致使自由基过度生成与聚集,氧化应激水平提高。急性跑台运动可显著增强骨骼肌Fas/FasL和Bax基因表达、降低Bcl-2基因表达和Bcl-2/Bax比率,使骨骼肌细胞发生凋亡,这可能与自由基过度聚集,氧化应激水平提高有关。 Objective: To measure oxidative stress as well as dynamic changes of Fas/FasL, Bcl-2/Bax gene expressions in skeletal muscle, and investigate the mechanism of apoptosis in skeletal muscle during acute treadmill exercise. Method: The different phase models of acute load treadmill exercise were setting up, and the mice were divid- ed into control group (Con), acute treadmill exercise for 45rain group (E45), 90rain group (E90), 120rain group (El20) and 150rain group (El50). Microplate reader was used to measure the levels of melondialdehyde (MDA), hydrogen peroxide(H202), nitric oxide(NO) and superoxide dismutase(SOD), quantitative real-time PCR technique was used to detect Fas/FasL, Bcl-2/Bax gene expressions in skeletal muscle.
出处 《中国康复医学杂志》 CAS CSCD 北大核心 2014年第1期20-25,共6页 Chinese Journal of Rehabilitation Medicine
基金 国家自然科学基金资助项目(31171142 30871212) 烟台大学青年基金资助项目(TY12Z08)
关键词 骨骼肌 氧化应激 细胞凋亡 急性跑台运动 Fas FasL Bcl-2 Bax keletal muscle oxidative stress apoptosis acute treadmill exercise dynamic change Fas/FasL Bcl-2/ Bax
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参考文献22

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