天狼猩红-偏振光法检测子宫内膜异位症中Ⅰ、Ⅲ型胶原

Detection of type Ⅰ,Ⅲ collagen in endometriosis implant by picrosirius - polarization

探讨子宫内膜异位症中Ⅰ、Ⅲ型胶原的含量和作用。方法：对子宫内膜异位症（内异症）２０例中的异位内膜组织红色病变期１２例（Ａ组）、黑色病变期８例（Ｂ组）和１１例非子宫内膜异位症患者的在位子宫内膜组织（Ｃ组）用天狼猩红染色，偏振光观察，ｐｈｏｔｏｓｈｏｐ分析，半定量研究Ⅰ、Ⅲ型胶原的含量。结果： Ｉ、Ⅲ型胶原在Ｃ组中含量最低（Ｐ＜０．０５），Ⅲ型胶原在Ａ组中含量最高（Ｐ＜０．０５），Ｉ型胶原在Ｂ组中含量最高（Ｐ＜０．０５）。Ｉ、Ⅲ型胶原在增生期和分泌期差异无显著性（Ｐ＞０．０５）。结论：Ⅰ、Ⅲ型胶原的异常沉积可引起内异症患者腹腔内发生异常的免疫反应。

Objective:To determine the type collagen presented in ectopic endome- trium and in the connective tissue surrounding ectopic endomtrial implant and to value their concentration and function. Method: The tissues of eleven cases with normal eutopic endome- trium(group C), 12 patients with red lesion (group A) and 8 patients with black lesion (group B) were collected and type Ⅰ,Ⅲ collagen were detected by sirius red and polarization micros- copy. The percentage of the stained area in the tissue was measured with a computerized image analysis system(pohotoshop). Results: There was significant difference among the three groups. The lowest concentration of type collagen was group C (P < 0.05). Then in group A, the concentration of type collagen was the highest among the three groups (P< 0. 05). Group B had the highest concentration of type collagan I (P < 0.05). Conclusions:Abnormal concentration of type collagen may induce abnormal immunoreaction in endometriosis.