慢性砷暴露对雌鼠子宫和卵巢组织雌激素受体结合位点相关抗原9和雌激素反应指蛋白mRNA表达的影响

Effects of chronic arsenic exposure on mRNA expression of estrogen receptor-binding fragment-associated gene 9and estrogen-responsive finger protein in female rat＇s uterus and ovary tissue

目的观察慢性砷暴露对雌鼠子宫和卵巢组织雌激素受体结合位点相关抗原9（Ebag9）和雌激素反应指蛋白（efp）mRNA表达的影响。方法将50只雌性Wistar大鼠按体质量随机分成5组，每组10只，分别饮用含砷量（三氧化二砷，As2O3）为0．00（对照）、0．05、0．10、0．20、0．40mg／L的水溶液，31周后通过实时定量PCR方法检测大鼠子宫和卵巢组织中Ebag9和efpmRNA的表达。结果对照、0．05、0．10、0．20、0．40mg／L组大鼠子宫组织Ebag9和efpmRNA表达量分别为0．761±0．178、0．521±0．130、0．544±0．035、0．525±0．198、0．498±0．240和0．795±0．171、0．874±0．077、0．797±0．066、0．796±0．040、0．832±0．096。与对照组比较，染砷各组Ebag9mRNA表达呈降低趋势，但差异无统计学意义（P均〉0．05），其中0．40mg／L组P值为0．055；与对照组比较，染砷各组efpmRNA表达呈升高趋势，但差异无统计学意义（P均〉0．05）。对照、0．05、0．10、0．20、0．40mg／L组大鼠卵巢组织Ebag9和efpmRNA表达量分别为0．702±0．484、0．719±0．336、0．693±0．095、0．706±0．055、0．728±0．073和0．924±0．061、1．009±0．034、0．930±0．085、0．929±0．068、1．012±0．101。0．05、O．20、0．40mg/L组Ebag9mRNA表达呈升高趋势，但与对照组比较，差异无统计学意义（P均〉0．05）；染砷各组efpmRNA表达均呈升高趋势，其中0．05、0．d0mg／L组与对照组比较，差异有统计学意义（P均〈0．05）。结论慢性砷暴露可引起雌性大鼠卵巢组织efomRNA表达改变。砷可能对雌性大鼠卵巢组织存在内分泌干扰效应。

Objective To observe the effects of chronic arsenic exposure on mRNA expression 0f estrogen receptor-binding fragment-associated gene 9（Ebag9） and estrogen-responsive finger protein（efp） in uterus and ovary of female rats. Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic （As203） concentrations given through drinking-water： 0.00 （control）, 0.05, 0.10, 0.20, 0.40 mg/L arsenic exposure groups and real-time RCR （RT-PCR） was used to detect the mRNA expression of Ebag9 and efp in uterus and ovary tissue at the 31 weeks of experiment. Results The mRNA expression levels of Ebag9 and efp of the 0.00, 0.05, 0.10, 0.20, 0.40 mg/L arsenic exposure groups were respectively as follows： 0.761 ± 0.178, 0.521 ± 0.130, 0.544 ± 0.035, 0.525 ± 0.198, 0.498 ± 0.240 and 0.795 ± 0.171, 0,874 ± 0.077, 0.797 ± 0.066, 0.796 ± 0.040, 0.832 ± 0.096. Compared with control group, a decreased tendency Was observed in Ebag9 mRNA level（with P value 0.055 in 0.40 mg/L arsenic exposure group） and increased tendency in efp mRNA level in experimental groups （all P 〉 0.05 ）. The mRNA expression levels of Ebag9 and efp in ovary of the five groups were by turns： 0.702 ± 0.484, 0.719 ± 0.336, 0.693 ± 0.095, 0.706 ± 0.055, 0.728 ± 0.073 and 0.924 ± 0.061, 1.009 ± 0.034, 0.930 ± 0.085, 0.929 ± 0.068, 1.012 ± 0.101. Compared with control group, the expression level of Ebag9 mRNA showed a increased tendency in 0.05, 0.20, 0.40 mg/L arsenic exposure groups（all P 〉 0.05）. The efp mRNA level increased in experimental groups, with significant difference in 0.05, 0.40 mg/L groups （all P 〈 0.05）. Conclusions The expression of efp mRNA has changed in ovary of female rats exposed to chronic arsenic. Arsenic may act as an environmental endocrine disruptor to exert its effect.