SRp20对口腔癌细胞丙酮酸激酶前体mRNA可变剪切的调控作用

SRp20 Regulates the Alernative Splicing of Pyruvate Kinase Pre-mRNA in Oral Squamous Carcinoma Cells

目的:研究SRp20参与口腔癌细胞中丙酮酸激酶前体mRNA可变剪切的调控机制。方法:采用siRNA在口腔癌细胞Cal27和Tca8113中沉默SRp20的表达,再以RT-PCR检测丙酮酸激酶的M1和M2亚型的表达水平。结果:SRp20的特异性siRNA转染Cal27和Tca8113细胞,显著降低了SRp20的表达水平。在Cal27细胞和Tca8113细胞中,抑制SRp20的表达后,M2的表达水平下降,M1与M2表达水平的相对比例显著增加。结论:在口腔癌细胞中,SRp20调控丙酮酸激酶前体mRNA可变剪切,利于M2亚型丙酮酸激酶的表达。

Objective： To investigate the function of SRp20 in the alternative splicing of pyruvate kinase in oral squamous carcinoma cells. Methods： The expression of SRp20 was suppressed by siRNA （small interfering RNA） in Ca127 or Tca8113 cells. The expression levels of pyruvate kinase M1 and M2 were analyzed by RT-PCR. Results： SRp20 siRNA efficiently clown-regulated SRp20 ex- pression in Ca127 and Tca8113 cells. Knockdown of SRp20 decreased M2 and increased the rela- tive ratio of M1/Mz. Conclusion： SRp20 regulates the alternative splicing of pyruvate kinase pre- mRNA and increases the expression of pyruvate kinase M2 in oral squamous carcinoma cells.