摘要
目的 :探讨免疫抑制因素 TNF- α在白血病发病中的作用。方法 :在大系列 MDS细胞遗传学研究基础上 ,应用酶联免疫吸附法 (EL ISA)对 2 3例 MDS、17例 AA、10例 AL 和 8例 ITP等患者骨髓液进行 TNF- α活性水平的测定。结果 :MDS TNF- α活性水平有明显增加、AL 则有显著性增加 (P<0 .0 5 )并同它们中染色体核型异常和 SCD阴性检出率的增加相一致。AA与正常人差别不明显 ,ITP与正常人几乎无差别 ,这同它们中大多数患者骨髓细胞核型正常和 SCD阳性的结果相一致。部分病例随访结果显示 ,当 MDS转白血病时不仅其骨髓细胞 SCD由阳性转阴性 ,其 TNF- α活性水平呈数倍于正常值的扩增。结论 :尽管 MDS和 AA同属造血干细胞病并有共同的遗传背景和发病早期 ,但前者主要还与体液免疫抑制因素 (TNF- α)相关 ,而后者则还与细胞免疫抑制因素 (如 T-淋巴细胞 )相关 ,因而有不同的表现。
Objective:To study the activity of TNF α in MDS and AA. Methods:There were analysed cytogenetically including karyotypic analysis and SCD assay in a large series of MDS and AA.Among them TNF alpha activity of BM serum were detected by using ELISA in 23 MDS,17 AA,10 AL and 8 ITP.Results:The significant and moderately increased TNF alpha activity were found in AL( P <0.05) and MDS( P =0.1),which were related to their higher percentages of patients with cytogenetic abnormalities,while normal and lower TNF alpha activity were found in ITP( P =0.7) and AA( P =0.4),which were related to their zero and lower percentages of ones.Amplificated activity of TNF alpha in 4 patients,which were followed up for 3～5 months,indicated relationship between amplification of TNF alpha activity and leukomogenesis. Conclusion:The different cellularity of both MDS AA may be due to different immunologic mechanism.The model of development of AL,MDS and AA was proposed,in which the related genes were showed.
出处
《白血病》
2000年第6期334-337,共4页
