红花黄色素对大鼠脑缺血再灌注损伤的保护作用

Protective effect of safflower yellow pigment on cerebral ischemia reperfusion injury in rats

目的探讨红花黄色素对大鼠脑缺血再灌注损伤的保护作用。方法 36只SD大鼠随机分为假手术组、模型组和治疗组各12只,治疗组术前腹腔注射红花黄色素10mg/(kg·d),假手术组、模型组均腹腔注射生理盐水2mL/d,连续7d。采用线栓法制作大鼠局灶性脑缺血再灌注损伤模型,术后48h行神经功能缺损评分,比较3组脑梗死率、脑组织含水率及丙二醛(malondialdehyde,MDA)、血浆超氧化物歧化酶(superoxide dismutase,SOD)、一氧化氮(nitric oxide,NO)水平变化。结果模型组脑组织含水率(86.2±5.9)%较假手术组(73.7±4.1)%明显增加(P<0.05);治疗组神经功能缺损评分(2.3±0.4)分、脑梗死率(8.6±3.2)%、脑组织含水率(79.1±5.0)%均较模型组(3.4±0.6)分、(13.9±4.5)%、(86.2±5.9)%明显降低(P<0.05);模型组大鼠脑组织中MDA、NO水平较假手术组明显增加(P<0.05),SOD活性明显降低(P<0.05);治疗组大鼠脑组织中MDA、NO水平较模型组明显降低(P<0.05),SOD活性明显增高(P<0.05)。结论红花黄色素可能通过降低脑组织中MDA、NO水平,升高SOD水平而发挥对大鼠脑缺血再灌注损伤的保护作用。

Objective To study the protective effect of safflower yellow pigment on cerebral ischemia reperfusion injury in rats. Methods Thirty-six rats were divided into 3 groups： sham operation group, model group and treatment group, with 12 rats in each group. Before operation, treatment group received intraperitoneal injection of safflor yellow （10 mg/（kg, d））, and sham operation group and model group were given intraperitoneal injection of normal saline （2 mL/d）, totally for 7 days. Rat focal cerebral ischemia models were established by using suture methed. Neurological function defect score was evaluated 48 hours after operation. The incidence rate of cerebral infarction, brain tissue water content, and the levels of malondialdehyde （MDA）, superoxide dismutase （SOD） and nitric oxide （NO） were detected and compared. Results The brain tissue water content was significantly higher in model group than that in sham operation group （P^0.05）. The neurological function defect score, the incidence rate of cerebral infarction and brain tissue water content were significantly lower in treatment group （2.3!0.4, （8.6＋3.2） ~, （79.1_--45.0） %） than those in model group （3.4--4-_0.6, （lg. 9＋4.5） ~ , （86.2＋_ 5.9） ~） （P^0.05）. The levels of MDA and NO in brain tissue were higher, and SOD activity decreased in model group in comparison with sham operation group （P^0.05）. Compared with model group, the levels of MDA and NO in brain tissue decreased and SOD activity increased in treatment group （P〈0.05）. Conclusions Safflower yellow pigment has obvious protective effect on rats with cerebral ischemia reperfusion injury by decreasing the levels of MDA and NO and increasing SOD activity in brain tissue.