海洛因诱导条件性位置偏爱大鼠顶叶联络皮质遥测脑电分析

Telemetry EEG of Parietal Association Cortex in Heroin-induced CPP Rats

目的记录海洛因诱导条件性位置偏爱(CPP)大鼠顶叶联络皮质(PtA)区脑电变化,分析其与觅药行为之间的关系。方法大鼠PtA区脑立体定位电极埋藏,分成手术对照组和海洛因诱导组,后者制作海洛因依赖模型。利用CPP视频系统结合脑电无线遥测技术,记录各组大鼠在黑白箱停留、黑-白箱穿梭和白-黑箱穿梭时大鼠PtA区脑电变化,分析其各波百分比的差异。结果与手术对照组比较,海洛因诱导组大鼠黑、白箱停留时,遥测脑电各波百分比差异无统计学意义,但海洛因诱导组大鼠穿梭时,与其停留状态脑电比较,右侧PtA脑电δ波百分比增加,β波、β2波百分比降低(P<0.05,P<0.01),左侧PtA区脑电β波、β2波百分比也显示减少(P<0.05,P<0.01)。进一步将海洛因诱导组大鼠穿梭时脑电与手术对照组大鼠穿梭时脑电相比,海洛因诱导组大鼠仅在白-黑穿梭时,左、右侧PtA区δ波百分比降低、θ、α、α1波百分比增高(P<0.05),而黑-白箱穿梭时,PtA脑电百分比改变差异无统计学意义。结论海洛因诱导的CPP大鼠穿梭时PtA区脑电变化与停留状态下脑电有所不同,但这种穿梭时脑电的改变,可能与大鼠伴药箱觅药行为发生无关。

Objective To determine the relationship between EEG changes of parietal association cortex （PtA） and drug-seeking behaviors of heroin-induced conditioned place preference （CPP） rats. Methods Stereotaxic electrode was buried in the PtA of rats, which were then divided randomly into heroin-induced CPP group and operation-only control group. A CPP video system in combination with EEG wireless telemetry was used for recording PtA EEG and the behaviors of the rats-staying in black or white chamber of the video box; shuttling between black-white chambers or between white-black chambers. Results No significant difference in percentage of the telemetry EEG waves was found between the two groups of rats when they stayed in the black or white chambers. The heroin-induced CPP rats had increased percentage of δ waves （P〈0.05） on the right PtA and decreased percentage of β and β2 waves on both right and left PtA （P〈0.05） when they shuttled between two chambers. Compared with the operation-only controls, significant decrease in the percentage of δ waves on both left and right PtA and increase in θ, α and α1 waves were evident （P〈0.05） only when the heroin-induced CPP rats shuttled between white-black chambers. Conclusion EEG changes on PtA of heroin-induced CPP rats differ between staying and shuttling states. Such changes may not be associated with drug-seeking behaviors.