溃疡方对乙酸胃溃疡大鼠TFF1、EGF和EGFR的影响

Effects kui-yang-fang on TFF1,EGF and EGFR in acetic acid induced gastric ulcers of rats

目的探讨溃疡方对乙酸性胃溃疡大鼠黏膜三叶肽因子1(TFF1)、表皮生长因子(EGF)和表皮生长因子受体(EGFR)的影响。方法将50只SD大鼠随机分为5组:正常组、模型组、溃疡方低剂量组、溃疡方高剂量组、雷尼替丁组,制备模型,采集标本,比较各组大鼠胃溃疡指数,用逆转录聚合酶链反应(RT-PCR)法检测胃黏膜组织中TFF1、EGF及其受体EGFR mRNA的表达,免疫组化法检测胃黏膜TFF1、EGFR的含量。结果 RT-PCR法结果显示,与正常组比较,模型组TFF1、EGF水平降低,差异无统计学意义,溃疡方高剂量组及雷尼替丁组TFF1均显著升高,差异有统计学意义(P<0.01),溃疡方各剂量组及雷尼替丁组的EGF含量均高于正常组,差异有统计学意义(P<0.01),而模型组EGFR的含量较正常组升高,差异无统计学意义,但溃疡方各剂量组及雷尼替丁组升高更明显,差异有统计学意义(P<0.01);与模型组比较,溃疡方低剂量组、高剂量组及雷尼替丁组TFF1、EGF及EGFR的表达差异有统计学意义(P<0.01);免疫组化法结果显示,溃疡方各剂量组及雷尼替丁组TFF1及EGFR的表达均显著高于模型组,差异有统计学意义(P<0.01),尤其是溃疡方高剂量组。结论溃疡方对乙酸所致大鼠胃溃疡有防御和修复治疗作用,其作用机制可能是通过促进胃黏膜中TFF1、EGF和EGFR的表达有关。

Objective To investigate the effects of kui-yang-fang on trefoil factor 1 （TFF1）, epidermal growth factor （EGF） and epidermal growth factor receptor（EGFR） in acetic acid induced gastric ulcers of rats. Methods 50 rats were randomly divided into five groups. The rats of the gastric ulcer model were used by the icy acetic acid. After drug treatment for 14 days,the rats gastric mucosa was collected to compare to the ulcer index,then check the content of TFF1, EGF and EGFR with RT-PCR, and observe TFF1, EGFR with immunohistochemical staining. Results Compared to the control,the levels of TFF1 and EGF in model group decreased, and the difference was not statistically significant. The EGFR level of model group was higher than that of normal group with no statistically significant difference. But the increase of kui-yang-fang groups and ranitidine group was more obvious, the difference was statistically significant （P 〈 0. 01 ）. Compared to the model group, kui-yang-fang and ranitidine groups significantly elevated the level of TFF1, EGF and EFR in gastric ulcer, the difference was statistically significant （ P 〈 0. 01 ）. Immunohistochemical staining showed that TFF1 and EGFR expression in kui-yang-fang and ranitidine groups were significantly increased, the difference was statistically significant （ P 〈 0. 01 ）, but the increase of kuiyang-fang of high-dose group was more obvious. Conclusion One of the possible mechanism is kui-yang-fang promotes expression of TFF1, EGF and EGFR to protect and repair gastric tissue.