胃癌血清和组织中Her-2/neu、MMP-2和Leptin的表达情况及临床意义

Expression and clinical significance of Her-2/neu,MMP-2 and Leptin in serum and tumor tissue of gastric carcinoma

目的检测Her-2/neu、基质金属蛋白酶-2(MMP-2)和瘦素(Leptin)在胃癌血清和组织中表达情况,探讨它们在胃癌发生、发展过程中的作用。方法分别应用ELISA和免疫组化(IHC)法检测胃癌血清和组织中Her-2/neu、MMP-2和Leptin表达情况。结果 Her-2/neu、MMP-2、Leptin在胃癌血清中阳性表达率明显高于在正常对照组中阳性表达率(P<0.05);Her-2/neu、MMP-2、Leptin在胃癌组织中阳性表达率明显高于在癌旁组织中阳性表达率(P<0.05)。Her-2/neu、MMP-2、Leptin在胃癌血清和癌组织中阳性表达率具有一致性。Her-2/neu、MMP-2和Leptin在胃癌组织中的阳性表达均与浸润深度、淋巴结转移和TNM分期显著相关(P<0.05),与患者的性别、年龄、肿瘤大小、肿瘤部位和肿瘤分化程度均无相关性。Her-2/neu在血清中表达与浸润深度、淋巴结转移和TNM分期呈显著正相关(P<0.05);MMP-2在血清中表达与淋巴结转移呈正相关(P<0.05);Leptin在血清中表达与病理特征无明显相关性。结论 Her-2/neu、MMP-2和Leptin在胃癌的发生、发展和转移过程中起促进作用,针对Her-2/neu、MMP-2和Leptin的检测可用于临床诊断,指导靶向治疗,并为临床预后提供科学合理参考。

Objective Detected Her-2/neu, matrix metalloproteinases-2 （MMP-2） and Leptin in stomach cancer serum and tissue, discussed their roles in occurance and development of gastric cancer. Methods Detected the ex- pression of Her-2/neu, MMP-2 and Leptin in serum by ELISA, and detected the expression of Her-2/neu, MMP- 2 and Leptin in stomach cancer tissue by IHC. Results The positive rates of Her-2/neu, MMP-2, Leptin in gastric cancer serum were higher than the positive rates in control group （ P 〈 0. 05 ）. The positive rates of Her-2/ neu, MMP-2, Leptin in gastric cancer tissues were higher than the positive rates in adjacent tissues （P 〈 0. 05 ）. The positive expressions of two tests had consistency. In tissues, the positive expression rates of Her-2/neu, MMP-2 and Leptin were correlated with the depth of invasion,lymph node metastasis, clinical stage （ P 〈 0. 05 ）. Their expression was not correlated with sex, age, size, site of tumor and the degree of differentiation. In serum, the positive expression of Her-2/neu was correlated with the depth of invasion, lymph node metastasis, clinical stage （P 〈 0. 05 ）. The positive expression of MMP-2 was correlated with lymph node metastasis （P 〈 0. 05 ）. The positive expression of Leptin had no significant correlation with pathological features. Conclusion Her-2/neu, MMP-2 and Leptin promote the occurrence, development and transfer process of gastric cancer. It can be used for clinical diagnosis, guide the targeted therapy and provide scientific and reasonable reference for gastric cancer prognosis.