BDNF rs12273539基因多态性与2型糖尿病认知障碍的关联存在性别差异

Gender differences between polymorphisms of BDNF-rs12273539 and cognitive impairment in T2DM patients

目的探讨脑源性神经营养因子(BDNF)rs12273539基因多态性和2型糖尿病认知障碍(T2DM-CI)之间的关联。方法选择T2DM-CI患者92例,无认知障碍的T2DM(T2DM-NCI)患者191例,聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法检测BDNF rs12273539基因多态性,SPSS 15.0及在线遗传软件SHEsis进行统计分析。结果在总体样本和男性样本中,BDNF rs12273539 TT基因型在认知障碍病例组分布相对过多,可能是T2DM-CI的易感基因型,在女性样本中BDNF-rs12273539等位基因和基因型分布在T2DM-CI组和T2DM-NCI组的差异无统计学意义。结论BDNF-rs12273539可能是T2DM-CI的易感基因;BDNFrs12273539基因多态性与T2DM-CI的关联存在性别差异。

Objective To investigate the genetic associations between polymorphisms of BDNF-rs12273539 and cognitive impairment in type 2 diabetes （T2DM） patients. Methods Participants were divided into two groups： T2DM patients complicated with cognitive impairment （T2DM-CI） group （n = 92） and type 2 diabetes patients without cognitive impairment （T2DM-NCI） group （n = 191）. Polymorphic SNPs of rs12273539 in the BDNF gene were genotyped using PCR-RFLP technology. The description of data, case-control analysis and association analysis between SNPs were performed with the SPSS 15.0 program and online SHEsis program. Results In the total samples and male samples, there was also a significant difference in BDNF rs12273539 genotype distribution between the patients and control groups （Х^2 =7. 639 4, P =0. 021 9;Х^2 =6. 643 0, P =0. 036 1, respectively）. In the female samples, no significant association with T2DM-CI was observed between two groups （ P 〉 0.05 ）. Conclusion According to the present results, it can be concluded that： （1） the genetic polymorphisms of BDNF gene are like- ly to confer susceptibility to T2DM-CI; （2） there are BDNF gene sex-specific genetic components involved in the pathology of T2DM-CI.