痰瘀同治方对小型猪痰瘀互结证冠心病血液流变性及血脂的改善作用

Effect of formula of removing both phlegm and blood stasis in improving hemorheology and blood fat of mini-swine with coronary heart disease of phlegm-stasis cementation syndrome

目的:观察痰瘀同治方对痰瘀互结证冠心病中国小型猪血液流变性及血脂代谢紊乱的改善作用。方法:将36只中国小型猪随机分为正常对照组、模型组、舒降之组和痰瘀同治方高、中、低剂量组(2.0,1.0,0.5 g·kg-1),每组6只;除对照组外,其他各组均高脂饲料喂养2周后,采用介入技术球囊损伤冠状动脉左前降支内皮,术后继续高脂饲料喂养8周,制备小型猪痰瘀互结证冠心病模型。术后给药8周,观察各组动物血液流变学参数、血脂水平及心肌缺血程度和范围的变化。结果:与对照组比较,模型组血清TC,TG,LDL-C及VLDL-C水平均明显升高(P<0.01),5 s-1和60 s-1切变率下的全血黏度均明显增加(P<0.01),心肌缺血程度和范围也明显增加(P<0.01);与模型组比较,痰瘀同治方组的心肌缺血程度和范围均明显减少(P<0.01),血清TC,TG,LDL-C及VLDL-C水平均明显降低(P<0.05或P<0.01),5 s-1和60 s-1切变率下的全血黏度均明显降低(P<0.05)。结论:痰瘀同治方能够改善痰瘀互结证冠心病中国小型猪血液流变学的异常状态,同时具有调节血脂的作用,对痰瘀互结证冠心病有一定的疗效。

Objective： To observe effect of formula of removing both phlegm and blood stasis （TYTZ） in improving hemorheol- ogy and blood fat of mini-swine with coronary heart disease of phlegm-stasis cementation syndrome. Method： Thirty-six Chinese mini- swine were randomly divided to six groups： the normal control group, the model group, the Shujiangzhi group and TYTZ groups with doses of 2.0, 1.0 and 0. 5 g · kg^- l, with six mice in each group. Except for the normal control group, all of other groups were fed with high-fat diet for 2 weeks. Interventional balloons are adopted to injure their left anterior descending artery endothelium. After the operation, they were fed with high-fat diet for 8 weeks to prepare the coronary heart disease model of phlegm-stasis cementation syn- drome. In the 8th week after the operation and administration, the changes in hemorheological parameters, serum lipid level, myocar- dial ischemia level and range were observed. Result： Compared with the normal control group, the model group showed significant in- crease in serum TC, TG, LDL-C and VLDL-C levels （ P 〈 0. 01 ）, whole blood viscosity under the shear rate of 5 s - 1 and 60 s - 1 （ p 〈 0. 01 ）, and myocardial isehemia degree and range （ P 〈 0. 01 ）. Compared with the model group, TYTZ groups revealed significant de- crease in myocardial ischemia degree and range （ P 〈 0.01 ）, serum TC, TG, LDL-C and VLDL-C levels （ P 〈 0. 05 or P 〈 0. 01 ） and whole blood viscosity under the shear rate of 5 s - 1 and 60 s - 1 （ p 〈 0. 05 ）. Conclusion： TYTZ could improve the abnormal hemorheol- ogy in Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome, and regulate serum lipid, with a certain efficacy for coronary heart disease of phlegm-stasis cementation syndrome.