深圳市一般人群HGV和TTV感染的研究

A Study on Hepatitis G Virus(HGV) & Transfusion Transmitted Virus(TTV) Infection in General Population of Shenzhen,

为探讨深圳市一般人群中HGV和TTV感染状况及其影响因素。方法采用随机抽样法选取研究对象 ,对HGV感染的检测先用ELISA法检测样本中的抗 -HGV ,对其中阳性样本再用反转录PCR(RT -PCR)进行检测 ;TTVDNA则采用巢式PCR方法检测。结果表明 ,深圳市一般人群中HGVRNA和TTVDNA阳性率分别为 2 33%和 14 77% ,男女阳性率HGVRNA为 2 4 5%和2 2 0 % ,TTVDNA阳性率为 17 86%和 12 0 %。年龄组间HGVRNA及TTVDNA阳性率差异均无显著性 ;单因素和Logistic回归分析未显示肝炎病史、近期手术史、注射史、拔牙史及乙型肝炎疫苗接种史等因素与HGV及TTV感染有关 ;HBsAg、抗 -HBS和抗 -HBc与HGV及TTV感染无统计学意义。不同职业人群中HGVRNA阳性率以中学生和教师较高 ;TTVDNA阳性率则以税务干部和教师高于其他人群 ,因此证明 ,深圳市一般人群中HGV和TTV感染率较高 。

Objective:To explore prevalence of hepatitis G virus(HGV) & transfusion transmitted virus(TTV) infection and their risk factors among the general population in Shenzhen Methods: According to the cluster sampling,1843 subjects were selected randomly and tested for anti-HGV by ELISA;and the positive samples were further tested for HGV RNA using RT-PCR; and tested for TTV DNA by nPCR. Results: The prevalence rate of HGV RNAwas2 33%(2 45%for male and 2 20%for female,respectively);and the prevalence rate of TTV DNA was 14 77%(17 86%for male and 12 0%for female,respectively). There was no significant difference between males and females,nor with different age groups.The histories of hepatitis,surgical dental operations and injections were not associated with HGV and TTV infection. Hepatitis B vaccination and positivity of HBsAg,anti-HBs and anti-HBc antibodies were not related to HGV RNA and TTV DNA prevalence rate.However ,HGV RNA prevalence rate was higher in middle school students and teachers than other professional groups;and TTV DNA prevalence rate was higher in tax collectors and teachers than others. Conclusion: The prevalence rates of HGV RNA and TTV DNA seemed to be high among the general population in shenzhen,but the risk factors of HGV and TTV infecton in the population need to be further studied.