银翘柴桂颗粒体内抗甲Ⅰ型流感病毒作用研究

Anti-influenza A Virus Effect of Yinqiao Chaigui Granula in Vivo

【目的】观察银翘柴桂颗粒的体内抗甲Ⅰ型流感病毒作用。【方法】选用NIH小鼠,随机分为正常组,模型组,阳性对照药组(利巴韦林,100 mg·kg-1·d-1),银翘柴桂颗粒高、中、低剂量组(剂量分别为6、3、1.5 g·kg-1·d-1);建立甲Ⅰ型流感病毒鼠肺适应株FM1人工感染NIH小鼠模型,以肺湿干比率、死亡保护率及肺组织病理变化为主要评价指标,观察银翘柴桂颗粒对感染甲Ⅰ型流感病毒鼠肺适应株FM1小鼠的防治效果。【结果】模型组小鼠肺湿干比率和死亡率显著升高,与正常组比较差异均有统计学意义(P<0.01),肺组织出现明显炎性病变;银翘柴桂颗粒高、中、低剂量组均可显著降低模型小鼠肺湿干比率和死亡率,与模型组比较差异均有统计学意义(P<0.05或P<0.01),并可改善感染小鼠的肺组织炎性病变,作用与阳性对照药利巴韦林相仿。【结论】银翘柴桂颗粒具有一定的体内抗甲Ⅰ型流感病毒作用。

Objective To investigate the effect of Yinqiao Chaigui Granula on counteracting influenza A virus in vivo. Methods NIH mice were randomized into normal control group, model group, positive control group （treated with virazole 100 mg·kg-1·d^-1）, and high-, middle- and low-dose Yinqiao Chaigui Granula groups （at the dosage of 6, 3 and 1.5 g·kg-1·d^-1, respectively） . The NIH mouse model of viral pneumonia was established by infection with pulmonary adaptive influenza A virus strain FM1. Lung wet-to-dry weight ratio, death rate, and pathological changes of lung tissue were observed to investigate the effects of Yinqiao Chaigui Granula on preventing and treating the infected mice. Results Lung wet-to-dry weight ratio and death rate were significantly increased in model group （P 〈0.01 compared with those in the normal control group） and the inflammatory changes of lungs were shown obviously. Yinqiao Chaigui Granula had obvious effect on decreasing lung wet-to-dry weight ratio and death rate （P〈0.05 or P〈0.01 compared with the model group） , and on relieving the lung inflammatory changes, the effects Yinqiao Chaigui Granula exerts certain anti-influenza A being similar to those of virazole group. Conclusion virus effect in vivo.