摘要
目的比较序列特异性引物-聚合酶链式反应(SSP-PCR)法和流式细胞术(FCM)法两种方法对人类白细胞抗原-B27(HLA-B27)的检测结果,为实验室检测提供参考。方法将100份待测标本分别采用SSP-PCR法和FCM法检测HLA-B27,用χ2检测统计这两种方法的结果有无差异。结果两种方法检测结果差异无统计学意义(P>0.05)。结论 SSP-PCR法与FCM法均适用于实验室对HLA-B27的检测。
Objective To compare the detection result of HLA-B27（human leukocyte antigen-B27） by the SSP-PCR（polymerase chain reaction sequence specific primers） and the FCM（flow cytometry）, in order to provide a reference for laboratory testing. Methods We collected the whole blood of 100 patients, and statistically analyzed the results of HLA-B27 by SSP-PCR and FCM. Results The results of two methods had no statistically significant differences （P〉0.05）. Conclusion Both the SSP-PCR and FCM methods are suitable for the laboratory diagnosis of the HLA-B27.
出处
《国际检验医学杂志》
CAS
2014年第1期70-71,74,共3页
International Journal of Laboratory Medicine
关键词
HLA抗原
脊柱炎
强直性
聚合酶链反应
human leukocyte antigen-B27
spondylitis, ankylosing
polymerase chain reaction
