摘要
以隶属于44科的60种药用植物为试材,以无水乙醇作为提取溶剂,采用生长速率法和孢子萌发法对选取的药用植物提取物进行抑菌活性的测定,以筛选出对葡萄炭疽病菌具有抑菌活性的药用植物资源。结果表明:在供试质量浓度为100mg/mL时,对葡萄炭疽病菌孢子萌发抑制率和菌丝生长抑制率均在80%以上的有11种植物提取物,抑制率超过90%的药用植物有艾蒿、苍耳、丁香、黄芩、苦参、肉桂和石菖蒲7种,其中丁香、肉桂和石菖蒲的提取物对孢子的萌发抑制率和菌丝生长抑制率均为100%。筛选试验结果表明,药用植物丁香、肉桂和石菖蒲具有开发成防治葡萄炭疽病新型植物源杀菌剂的潜力。
Taking 60 medicinal plant species belonging to 44 families in China as materials, ethanol as extracnon solvent, their antifungal activity against grape anthracnose disease were studied using spore germination method and mycelium growth rate method in vitro,in order to study antifungal activities of medicinal plant extracts against grape anthracnose disease and provide basis for exploitation of hio-fungicide from plant resource. The results showed that at concentration of 100 mg/mL, inhibition rate of plant extracts on the mycelia growth and conidial germinations of 11 plant species presented more than 80%, respectively. Among them, Artemisia argyi, Xanthium sibiricum, Syzygiurn aromaticum, Scutellaria baicalensis , Sophora flavescens , Cinnamomum cassia, Acorus tatarinowii with inhibition rate over 90%. Particularly, the extracts of Syzygium aromaticurn, Cinnamomum cassia and Acorus tatarinowii presented inhibition rate of the mycelia growth and conidial germinations were 100%, respectively. According to the above results, Syzygium arornaticurn ,Cinnarnomurn cassia and Acorus tatarinowii were chosen as the products for exploitation the botanical fungicide against the grape anthracnose disease.
出处
《北方园艺》
CAS
北大核心
2014年第2期119-123,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(30973963)
安徽高校省级自然科学研究资助项目(KJ2012B168
KJ2013A234)
安徽省淮北市科技计划资助项目(HB070141
HB080227)
关键词
葡萄
炭疽病
药用植物
抑菌活性
grape
grape anthracnose disease
medicinal plants
antifungal activities