丹参酮对高血压大鼠心脏保护作用

Effects of tanshinone on hypertensive rat heart protection

目的:通过建立肾性高血压大鼠模型,观察高血压大鼠心脏形态及NOS活性和NO产量变化,来探讨丹参酮ⅡA(TanshinoneⅡA,TSN)对心脏的保护机制。方法:应用经典的双肾双狭复制肾血管性高血压大鼠模型(two～kidney,two～clip renovascular hypertension model,2K2C RH model),设立假手术组、高血压组、丹参酮治疗组。采用鼠尾测压法测定大鼠血压;分别应用化学比色法法和硝酸还原酶法测定心肌组织NOS活性测定及一氧化氮(NO)的浓度;结果:丹参酮可促进心肌组织NOS活性及NO产量增加,并可明显改善RH大鼠心肌肥大和间质纤维化。结论:丹参酮可明显改善RH大鼠心肌肥大和心肌间质纤维化,此作用可能与TSN促进心肌组织eNOS蛋白表达、上调NOS活性和NO产量有关。

Objective：To investigat the changes in nitric oxide （ NO） content and nitric oxide synthase （ NOS） activity in the myocar-dium of renovascular hypertensive rats and explore the effects of Tanshinone ⅡA on protective mechanism of heart .Methods：The renovas-cular hypertension was induced by 2-kidey-2-clip on Wistar rats.Rats were divided into 3 groups：sham-operated group, hyperten-sive group, TSN-treated group.Blood pressure was measured by rat tail baumanometer .Levels of NO and eNOS activity level were meas-ured by colorimetry .Heart tissue sections were stained with HE method .Results：Tanshinone could promote NOS activity and NO content increased myocardial tissue , and may significantly improve the treatment of myocardial hypertrophy and interstitial fibrosis in rats .Conclu-sion：Tanshinone could obviously improve the myocardial hypertrophy and myocardial interstitial fibrosis in HP rats , which may be ex-pressed, and TSN promotes myocardial eNOS protein upregulation of NOS activity and NO production .