河南西南部女性宫颈癌高危优势HPV基因型分布及年龄特征分析

Analysis of the distribution of high-risk HPV genotypes and age characteristics of female cervical cancer in Southwest Henan

目的:分析河南西南部高危HPV基因型的分布及年龄特征,寻找优势基因型。方法:临床常规收集具有宫颈病变的宫颈脱落细胞,DNA抽提,荧光定量PCR进行分析,数据输入SPSS11.5,分析基因分布特征,确定优势基因型。结果:检测宫颈脱落细胞共468份,高危HPV检出127份,阳性率为27.1%。高危HPV基因型中,排列前5位的基因型依次为58(24.87%)、16(16.58%)、56(12.59%)、52(8.80%)、18(8.29%),其中58型感染率最高,为优势基因型。感染类型以混合型为主,占69.58%。高危HPV感染率最高在34岁以下,差异具有统计学意义(P<0.05)。结论:河南西南部高危HPV感染以58型为优势基因,有其地域特征,为该地区在高危HPV感染的预防指导、病毒基因型检测及疫苗设计的进行奠定基础。

Objective： To analyze the genotypes distribution and age characteristics of the high - risk human papillomavirus （HPV） in Southwestern Henan, then look for dominant genotype. Methods： Cervical cells with cervical lesions were clinically routinely collected. DNA of cervical cells were extracted and quantitative PCR was used to analyzed. Finally, the datas were entered into SPSS11.5 to ana- lyze gene distribution characteristics and determine the dominant genotype. Results： In this study, 468 cases were sent to detect by quantitative PCR and 127 cases of high -risk samples were determined, the positive rate was 27.1%. The top five high -risk HPV genotypes were 58 （24. 87% ）, 16 （16. 58% ）, 56 （12. 59% ）, 52 （8.80%）, 18 （8.29%） in turn. The highest infection rate was HPV58 genotypes and it was dominant genotype of female in Southwestern Henan. Mixed genotypes infection were main, accounted for 69. 58%. High - risk HPV infection was the highest in 34 years or less, the difference was statistically significant （P 〈 0. 05） . Conclusion： HPV58 is the dominant gene for high - risk HPV genotype infection in Southwestern, with geographical features. This settles a foundation for high - risk HPV infection prevention guidelines, the virus genotyping and vaccine design.