CD147与MMP-2在葡萄胎组织中的表达及其意义

Expressions and significances of CD147 and MMP-2 in hydatidiform mole tissues

目的:探讨CD147、MMP-2在葡萄胎组织中的表达及两者间的相互联系,预测葡萄胎恶变的可能。方法:应用免疫组织化学法对葡萄胎患者首次清宫术后40例组织标本(其中葡萄胎组31例,侵葡组6例,失访3例)进行CD147、MMP-2的检测,并与正常早孕患者绒毛组织作比较。再将葡萄胎高危组分为恶变组和未恶变组,应用免疫组织化学法检测CD147、MMP-2在含有高危因素的葡萄胎患者中的表达,分析其与葡萄胎组织恶变的关系。结果:①CD147主要表达于合体滋养层细胞以及增生滋养层细胞的胞膜,少量表达于胞浆;MMP-2主要表达于合体滋养层细胞以及增生滋养层细胞的胞浆,少量表达于胞膜。②CD147、MMP-2在侵蚀性葡萄胎组、葡萄胎组、正常绒毛组的表达呈依次递减的趋势,且两组间比较有统计学意义。③在含有高危因素的恶变组中,CD147、MMP-2平均灰度值较未恶变组的平均灰度值低(P<0.05)。④侵蚀性葡萄胎组、葡萄胎组、正常绒毛组中MMP-2与CD147的表达呈正相关。结论:①CD147、MMP-2在葡萄胎、侵蚀性葡萄胎发生发展过程中可能有促进作用,且有可能促进葡萄胎组织的恶变。②在含有高危因素的葡萄胎恶变患者中CD147、MMP-2的表达较未恶变组明显升高,提示联合检测CD147、MMP-2对预测葡萄胎的恶变具有一定的临床意义,有可能成为临床预测葡萄胎恶变的重要指标。

Objective： To explore the expressions and relationships of CD147 and MMP -2 in hydatidiform mole tissues and determine whether it can become an important index to predict the malignant change of hydatidiform mole. Methods： 40 cases were pathologically confirmed as hydatidiform mole. Immune histochemical method was applied to measure CD147, MMP -2 in the hydatidiform mole, including 31 cases of hydatidiform mole group, 6 cases of invasive hydatidiform mole, 3 eases lost to follow - up, and compared with 30 cases of patients with normal early pregnant villi tissue. Then hydatidiform mole malignant change high -risk group （33 cases） were divided into malig- nant group （6 cases）, not malignant group （27 eases）. Expressions of CD147 and MMP -2 and the relationships with hydatidiform mole tis- sues of malignant transformation were analyzed. Results： ①MMP - 2 was mainly expressed in syncytiotrophoblast cells, and cytotrophoblast cells, cytoplasm, a small amount of expression in syncytiotrophoblast cells, and cytotrophoblast cells, cytoplasm in cell membrane; CD147 was mainly expressed in syncytiotrophoblast cells, and cytotrophoblast cells, cytoplasm in cell membrane. ②CD147and MMP- 2 showed a decreasing trend in the expression of invasive hydatidiform mole group, hydatidiform mole group, normal villi group, and the difference between the two groups was statistically significant. ③The average gray value of CD147 and MMP - 2 in the malignant group with risk factors were less than those of without malignant group g average gray value , and the differences were statistically significant. ④ The expressions of MMP - 2 and CD147 in aggressive hydatidiform mole group, hydatidiform mole group and normal villi had certain correlation and were positively correlated. Conclusion： ①CD147, MMP -2 may have a role in the process and development of hydatidiform mole, and may promote the malignant transformation of hydatidiform mole. ②The expressions of CD147 and MMP - 2 in malignant group are obviously higher than those of without malignant group, which indicate that the combined detection of CD147 and MMP - 2 has clinical significance in predicting malignant transformation in hydatidiform mole and might become clinically important predictor of hydatidiform mole malignant change.