餐厨废水中A型产气荚膜梭菌的分离与鉴定

Isolation and identification of Clostridium perfringens type A in the wastewater from dining kitchen

为了对餐厨废水中的产气荚膜梭菌进行鉴定,试验采用TSC培养基进行厌氧培养、显微镜检等常规方法进行分离,并提取细菌基因组,设计、合成产气荚膜梭菌α、β、ε与ι毒素基因引物,配制PCR反应体系并设置反应条件,对扩增产物进行电泳检测。结果表明:分离菌在TSC培养基上产生黑色菌落,经琼脂糖凝胶电泳只有α毒素基因得到良好扩增。说明此次分离菌为A型产气荚膜梭菌。

To isolate and indentify Clostridium pegCringens in the wastewater from dining kitchen, the conventional methods including TSC anaero- bic culture and microscopy and so on, were used to extract bacterial genome. The a - , b - , ε - and t - toxin gene primers of Clostridium per- fringens were designed and synthesized, and then the PCR reaction system was prepared with proper reaction conditions. Finally, the amplification product was detected by eleetrophoresis. The results showed that black colonies appeared on the TSC medium, and the agarose gel eleetrophoresis showed that only alpha - toxin gene was amplified well. The results indicate that the isolate is identified as Clostridium pe^Cringens type A.