益生性植物乳杆菌C88的高密度培养条件优化研究

Studies on optimization of conditions for high cell density culturing of probiotic Lactobacillus plantarum C88

以MRS为基础培养基，对培养基的碳氮源和培养条件进行了优化，确定了植物乳杆菌C88优化培养基的配方为：果糖10g／L,葡萄糖20g／L，大豆蛋白胨26．66g／L，酵母浸粉13．33g/L，柠檬酸钠5g／L，无水乙酸钠5g／L，KMP042g／L，MgS04O2g／L，MnSO40．05g/L，吐温801．0mL／L。另外对植物乳杆菌C88高密度培养条件进行了优化，结果表明，植物乳杆菌C88在35℃条件下，同时流加20％的Na2CO3使发酵液pH值保持6．0-6．5，静止培养16h后，活菌数可达9．3×10^10mL^-1，本研究为植物乳杆菌C88冻干直投式发酵剂的制备奠定了基础。

Based on MR.S broth, the optimization medium composition and culture conditions for Lactobadllus plantarum C88 were studied. The optimized medium was obtained with fructose 10 g/L, glucose 20 g/L, soy peptone 26.66 g/L, yeast extract 13.33 g/L, sodium citrate 5 g/L, sodium acetate anhydrous 5 g/L, dipotassium phosphate 2 g/L, magnesium sulfate 0.2 g/L, manganese sulfate 0.05 g/L, tween 80 1 mL/L. The fermentation conditions were optimized using this enrichment medium, and the results showed that the viable count of lacto-bacillus plantarum C88 reached 9.3×10^10 CFU/mL under the conditions that the pH of medium was 6.0-6.5 adjusted with 20% Na2CO3, and cultivated statically at 35℃ for 16 h.