一测多评法同时测定五味子中6种木脂素类成分含量

Simultaneous Determination of Six Lignanoids in Schisandra chinensis by Quantitative Analysis of MultiComponents by Single Marker

摘要：目的建立一测多评法同时测定五味子中6个木脂素类成分含量的方法。方法高效液相色谱法，采用LichrosphereC18（4．6mm×250mm，5μm）色谱柱；流动相为乙腈一水梯度洗脱，流速1mL·min-1；柱温30℃；检测波长217am。以五味子醇甲为内参物，建立五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素与内参物的相对校正因子，并进行含量计算，实现一测多评。同时采用外标法测定12批五味子中6个成分的含量，比较计算值与实测值的差异，验证所建立方法的准确性。结果五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子乙素分别在0．09～1．62μg（r=0．9999）、0．0408～0．7344μg（r=0．9999）、0．0208～0．3744μg（r=0．9999）、0．0242—0．4356μg（r=0．9999）、0．0532—0．9576Ixg（r=0．9999）和0．0258—0．4644μg（r=0．9999）内线性关系良好。建立的相对校正因子重现性良好，采用校正因子计算的含量值与实测值之间无显著差异。结论所建立的方法准确、可行，可用于五味子质量评价。

OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker （QAMS） for simul- taneous determining six lignanoids in Schisandra chinens（S, chinensis）. METHODS A HPLC method was set up. Lichrosphere C18 column（4. 6 mm×250 mm,5 μm） was used. Acetonitrile-water was used as gradient mobile phase. The flow rate was 1.0 mL ~ min-t. The column temperature was 30 ℃ and detection wavelength was 217 urn. A method was developed for QAMS to determine schizandrol A, sehizandrol B, sehisantherin A, deoxyschizandrin, sehizandrin B, and sehizandrin C in S. chinensis. Schizandrol A was selected as internal standard; the relative correction factors （RCF） of sehizandrol B, schisantherin A, deoxyschizandrin, schizandrin B, and schizandfin C to sehizandrol A were calculated. The contents of the six lignanoids in 12 different batches of S. chinensis were determined by both external standard method and QAMS. The QAMS method was evaluated by comparison of its assay results with that of external standard method. RESULTS The linear range of sehizandrol A, schizandrol B, schisantherin A, deoxyschizandrin, schi- zandrin B, and sehizandrin C were within 0. 09 -1.62 μg（r =0. 999 9） , 0. 040 8 -0. 734 4μg （r =0. 999 9） , 0. 020 8 -0. 374 4μg（ r = 0. 999 9）, 0. 024 2 - 0. 435 6 μg（ r = 0. 999 9）, 0. 053 2 - 0. 957 6 μg（ r = 0. 999 9） and 0. 025 8 - 0. 464 4 μg（ r = 0. 999 9 ）, respectively. No significant differences were found between the quantitative results of external standard method and QAMS. CON- CLUSION The developed method is accurate, feasible, and can be used for quality evaluation of S. chinensis.