EB病毒LMP1在鼻咽癌细胞中对细胞因子分泌的影响

EB virus-encoded LMP1 mediate cytokines production in nasopharyngeal carcinoma cells

目的探讨EB病毒LMP1的分子致瘤机制,对LMP1是否促进IL1、IL2、IL3、IL4、IL6、IL8、IL10、TNF-α等细胞因子分泌进行研究。方法鼻咽癌细胞系CNE2和HNE2以LMP1的表达和对照质粒处理,利用Western blotting方法从蛋白水平上确定LMP1的表达;利用细胞因子的ELASA试剂盒测定细胞上清中IL1、IL2、IL3、IL4、IL6、IL8、IL10、TNF-α的浓度。结果鼻咽癌细胞在转染LMP1的表达质粒pcDNA3-LMP1后,细胞内的LMP1表达明显上调;在上调LMP1的细胞内,发现IL1、IL2、IL3、IL4的表达变化不明显(无统计学意义),在CNE2细胞中,转染组与转染对照组比较,IL6、IL8、IL10、TNF-α分别上调了6.7、4.6、3.6和3.4倍;在HNE2细胞中IL6、IL8、IL10、TNF-α分别上调了6.6、6.1、7.2和3.1倍(P<0.05)。结论鼻咽癌细胞系中LMP1对IL1、IL2、IL3、IL4的表达影响不明显,但明显促进IL6、IL8、IL10、TNF-α等细胞因子的分泌。

[Objective] To investigate the molecular tumorigenic mechanism of EB virus-encoded LMP1, on whether LMP1 promotes ILl, IL2, IL3, IIA, IL6, IL8, ILl0 and TNF-α secretion. [Methods] Transfected the LMP1 expression and control vector in nasopharyngeal carcinoma cell lines CNE2 and HNE2, used west ern blotting method to determine the expression of LMP1 from protein level, the supernatants of cells were analyzed using ELISA kit of ILl, IL2, IL3, IIA, IL6, IL8, ILIO, TNF-α for determination concentration of cytokine. [Results] After transfected pcDNA3-LMP1, LMP1 expression was significantly up-regulated in na- sopharyngeal carcinoma cells; in the up-regulation of LMP1 cells compare the control cells, which expression of ILl, IL2, IL3, IIA have not change significantly （not statistically significant）, but the secretion of IL6, IL8, ILl0, TNF-α were upregulated in the 6.7, 4.6, 3.6 and 3.4 times in the CNE2 cell; and the IL6, IL8, ILl0, TNF-α were upregulated in the 6.6, 6.1, 7.2 and 3.1 times in HNE2 cells （P〈0.05）. [Conclusion] EB virus-encoded LMP1 induces IL6, IL8, ILl0, TNF-α production in nasopharyngeal carcinoma cells, but not oromoted the secretion of ILl, IL2, IL3, IL4.