褪黑素联合顺铂对人食管癌Eca109细胞株的增殖抑制作用研究

The inhibiting effect of melatonin combined with cisplatin on the proliferation of Eca109 cell line

目的:观察褪黑素(Mel)联合顺铂(DDP)对人食管癌Eca109细胞增殖和凋亡的影响。方法:以DDP 2.5 mg/L、不同浓度Mel及两药联合作用于食管癌Eca109细胞。通过MTT实验检测用药后对食管癌Eca109细胞增殖的影响,并计算抑制率;通过集落克隆形成实验观察用药后对细胞集落克隆形成的影响;通过流式细胞仪检测用药后细胞凋亡率的变化。结果:DDP2.5 mg/L与不同浓度Mel联合用药组对食管癌Eca109细胞生长抑制率均明显高于单独使用DDP或Mel组(P<0.01);10-5mol/L Mel诱导人食管癌Eca109细胞的24 h凋亡率为7.8%,10-5mol/L Mel与2.5 mg/L DDP联合刺激人食管癌Eca109细胞的24 h凋亡率为32.0%,高于DDP本身诱导的24 h凋亡率9.7%(P<0.01)。结论:Mel可增强DDP对人食管癌Eca109细胞增殖的抑制作用,并增强其诱导凋亡作用,为临床食管癌的治疗提供了一定的实验研究基础。

Objective ：To explore the effects of melatonin combined with cisplatin on the proliferation and apoptosis of Ecal09 cells. Methods：Eca109 cells were co-cultured with different concentrations of melatonin combined with or without cisplatin 2.5 mg/L. The inhibiting effects on the proliferation of Eca109 Cell were examined using MTF assay, and the inhibitory rate was analyzed. The cloning cell colony formation was observed using colony clone forming experiment, and the cell apoptosis rate was detected using flow cytometry after treatment. Results：The inhibitory rates of Eca109 cells induced with different concentrations of melatonin combined with eisplatin 2.5 mg/L were significantly higher than that in Eca109 cells induced with cisplatin or melatonin alone （P 〈 0.01 ）. Melatonin combined with cisplatin could obviously enhanced the inhibitory effects on colony formation of Ecal09 cells. The apoptosis rate of Ecal09 cells induced with 10 -5 mol/L of Melatonin for 24 h was 7.8%. The apoptosis rate of Ecal09 cells induced with 10 -5 mol/L of Melatonin combined with 2.5 mg/L of cisplatin for 24 h was 32.0%, which was higher than that in cells induced with only cisplatin （9.7%） （P 〈0.01 ）. Conclusions：Melatonin can enhance the anti-proliferative and apoptosis-inducing effects of cisplatin on Eca109 cells, which provide the basis of clinical treatment for esophageal cancer.