CD133在乳腺癌组织中的表达及其与微血管密度的关系

The CD133 expression in breast cancer and its relationship with microvessel density

目的:检测肿瘤干细胞标志物CD133在乳腺浸润性导管癌(infiltrating ductal carcinoma,IDC)组织中的表达及其与微血管密度(microvessel density,MVD)的关系和临床病理意义。方法:采用免疫组织化学ElivisionTMplus法检测105例IDC组织和20例癌旁乳腺组织中CD133的表达以及MVD计数。结果:在癌旁乳腺组织和IDC组织中,CD133的阳性率分别为5.0%和60.0%,差异有统计学意义(P<0.01);CD133的表达水平在肿瘤组织的分化程度、淋巴结转移和临床分期间差异均有统计学意义(P<0.01);MVD计数在肿瘤患者是否有淋巴结转移以及临床分期高低间差异均有统计学意义(P<0.01和P<0.05);且CD133的阳性和阴性表达间MVD计数差异有统计学意义(P<0.01)。结论:CD133的表达和MVD计数与IDC组织的淋巴结转移与否和临床分期均有一定关系;CD133和MVD联合检测对IDC的进展及预后判断有重要意义。

Objective：To investigate the expression of CD133, a cancer stem cell marker, in infiltrating ductal carcinoma（IDC） and its relationship with microvessel density（MVD） and clinical significance. Methods： The CD133 expressions in 105 specimens of IDC and 20 specimens of normal breast tissue and MVD count were detected by immnnohistochemical ElivisionTM plus method. Results： The positive rates of CD133 expression in IDC and normal breast tissue were 5.0% and 60.0% ,respectively ,the difference of which had statistical significance（ P 〈 0.01 ）. The CD133 expression level differences in tumor differentiation, lymph node metastasis and clinical stage had statistical significance（P 〈 0.01 ）. The MVD count differences in lymph node metastasis and clinical stage had statistical significance（ P 〈0.01 and P 〈 0.05 ）, the difference of MVD count in the positive and negative specimens of CD133 expression had statistical significance（P 〈 0.01 ）. Conclusions：The CD133 expression and MVD count are related to tumor Lymph node metastasis and clinical stage. The joint detection of CD133 and MVD have important significance in predicting the progress and prognosis of IDC.