消化系统恶性肿瘤患者血清细胞质胸苷激酶的检测

The diagnostic significance of serum cytoplasmic thymidine kinase-1 in digestive system carcinoma

目的:探讨消化系统恶性肿瘤患者血清细胞质胸苷激酶(TK1)检查在临床应用上的意义。方法:利用免疫印迹增强化学发光法,检测144例消化系统肿瘤患者和60例健康体检者(对照组)的血清TK1水平,并进行统计学分析。结果:消化系统肿瘤患者TK1阳性率为59.0%,明显高于对照组的11.7%(P<0.01);腺癌阳性率72.7%,肝细胞癌阳性率68.6%,均较鳞癌的20.0%升高(P<0.05);胃癌、肝癌、胰腺癌及结肠癌TK1阳性率均较食管癌升高(P<0.05)。结论:TK1在消化系统恶性肿瘤患者中阳性率较高,尤其在胃、结肠和胰腺的腺癌和肝细胞癌患者中明显升高,提示TK1检测对消化系统腺癌有一定特异性。血清TK1高表达对于筛查和诊断胃肠道腺癌及肝细胞癌具有应用价值。

Objective：To investigate the clinical significance of serum cytoplasmic thymidine kinase-1 （TK1） in the diagnosis of digestive system carcinoma. Methods ：The serum levels of TK1 in 144 patients with digestive system carcinoma and 60 healthy people were detected by enhanced chemi-luminescence assay. The data were statistically analyzed. Results：The TK1 positive rates in the patients with digestive system carcinoma（ 59.0% ） were significantly higher than that in healthy people（ 11.7% ） （ P 〈 0. 01 ）. The TK1 positive rates in adenocarcinoma（ 72.7% ） and hepatoeytic carcinoma （68.6%） were higher than that in squamous cell carcinoma （20.0%） （P 〈 0.05 ）. The TK1 positive rates in gastric, hepatic,pancreatic and colonic carcinoma were higher than that in esophagal squamons cell carcinoma （ P 〈 0. 05 ）. Conclusions ： The TK1 positive rate is high in digestive system carcinoma, especially in the adenocarcinoma of stomach,colon and pancreas and hepatocytic carcinoma, which surgest that the serum TK1 level is specific to the digestive gland carcinoma. The high expression of serum TK1 has certain applicative value in the screening and diagnosis of gastrointestinal adenocarcinoma and hepatocytic carcinoma.