俄罗斯杂花苜蓿再生体系的建立

Establishment regeneration system of Russian variegated alfalfa

为实现对俄罗斯杂花苜蓿(Medicago sativa subsp.varia)优良种质资源的利用,从而为苜蓿遗传改良奠定基础,本研究以MS培养基为基本培养基,以俄罗斯杂花苜蓿无菌苗的子叶、下胚轴为外植体,研究不同生长调节物质种类与组合对愈伤组织诱导和分化的影响,为建立其再生体系作初步探索。结果表明,俄罗斯杂花苜蓿的下胚轴比子叶更适合诱导愈伤组织,其最佳诱导培养基为MS+1.0mg·L-1 2,4-D+0.5mg·L-1 6-BA+0.5mg·L-1 KT;而以子叶为外植体的最佳诱导培养基为MS+1.0mg·L-1 2,4-D+1.0mg·L-1 6-BA+1.0mg·L-1 NAA,最佳分化培养基为MS+0.5mg·L-1 KT+0.3mg·L-1 NAA,下胚轴和子叶愈伤分化率分别为83.3%和91.1%;生根成苗培养基为1/2MS+1.0mg·L-1 IBA,生根率达96.7%。

To realize the use of excellent germplasm resource and lay the foundation of alfalfa genetic improvement. This study took MS solid medium as basic medium and cotyledons and hypocotyls of Medi- cago sativa subsp, varia as explants to discuss the effects of different phytohormone types and combina- tions on callus induction and differentiation. The results showed that hypocotyls was more suitable to induce callus than cotyledons as the explant of Russian variegated alfalfa and the best induced medium with hypocotyls as the explant was MS＋1. 0 mg · L-1 2,4-D ＋0.5mg · L-1 6-BA＋0.5 mg · L-1 KT; The best induced medium with cotyledon as the explant was MS＋I. 0 mg · L 1 2,4-D＋1.0 mg · L-1 6-BA-J-1.0 mg · L -1 NAA. The best differentiation medium was 0.5 mg · L-1 KT ＋0.3 mg· L 1 NAA, and differen- tiantion ratios of hypocotyls and cotyledons were 83.3% and 91.1%, respectively. The optimal seedling formation medium was 1/2MS4-1.0 mg · L -1 IBA, and the ratio was 96.7%.