1-(3,4-二羟基苯甲酰基)芘-硼酸络合物对铜离子的双重光谱响应及其分析应用

Dual spectral response of 1-(3,4-dihydroxy benzoyl)pyreneboric acid complex to copper and its analytical application

采用荧光光谱法和分光光度法探讨了Cu2＋与1-（3,4-二羟基苯甲酰基）芘-硼酸络合物间的相互作用.结果表明,Cu2＋使1-（3,4-二羟基苯甲酰基）芘-硼酸络合物在379 nm和399 nm处的荧光猝灭,并且溶液颜色由无色变为浅黄色.在379 nm处,体系荧光强度变化与Cu2＋浓度呈良好的线性关系,线性范围为2.5×10-6～4.0×10-5 mol/L,方法的检出限为1.2×10-6 mol/L.方法用于环境水样中铜的测定,回收率为97％～99％.初步探讨了反应机理,认为Cu2＋与1-（3,4-二羟基苯甲酰基）芘的强结合能力使1-（3,4-二羟基苯甲酰基）芘-硼酸络合物分解,同时形成1-（3,4-二羟基苯甲酰基）芘-Cu2＋络合物,导致体系荧光光谱和吸收光谱均发生明显变化.

The interaction between Cu2＋ and 1- investigated by fluorescence spectrometry and （3,4-dihydroxy benzoyl） pyrene-boric acid complex was spectrophotometry. The results showed that Cu2＋ had fluorescence quenching effect on 1-（3,4-dihydroxy benzoyl） pyrene-boric acid complex at 379 nm and 399 nm and the color of its solution was changed from colorless to pale yellow. At 379 nm, the fluo- rescence intensity change showed a good linear relationship with Cu2＋ concentration with linear range of 2.5 ×10^- 6-4.0 × 10^-5 mol/L and detection limit of 1.2 × 10^-6 mol/L. The method was applied to the determination of copper in environmental water samples and the recoveries were 97%-99%. The reaction mechanism was tentatively discussed as that the strong binding ability of Cu2＋ with 1-（3,4-di- hydroxy benzoyl） pyrene caused the decomposition of 1-（3,4-dihydroxy benzoyl） pyrene-horic acid complex and simultaneous formation of 1-（3,4-dihydroxy benzoyl） pyrene-Cu2＋ complex, leading to an significant change in the fluorescence and absorption spectrum of its system.