乙醇超声提取毛冬青(Llex pubescens)茎中总黄酮的工艺研究

Study on Ultrasonic Extraction Process of Total Flavonoids from Pubescent Holly Stem Using Ethanol as the Extraction Solvent

目的:筛选乙醇提取毛冬青茎中总黄酮的最佳条件.方法:采用正交试验研究乙醇浓度、料液比、提取时间、提取次数对毛冬青茎中总黄酮提取的影响,用紫外分光光度法测定总黄酮含量.结果:乙醇作为提取溶剂的最佳工艺是乙醇浓度30%,料液比1﹕20,提取3h,提取3次,此条件下吸光值为0.529.结论:乙醇浓度影响最大,其次是提取次数,提取时间。

Objective： To optimize and establish the extraction of total flavones in Pubescent Holly stem using ethanol as the extraction solvent. Method： Orthogonal experiment was designed with the content of total flavonoids as index, and with ethanol concentration, the ratio of material and solvent, extracting time and frequency as factors. Results： When using ethanol as the extraction solvent, 30% ethanol concentration, solid to liquid ratio of 1：20, extraction time was 3h, extracting 3 times, at this time was the best extraction effect; the OD500 was up to 0.529. Conclusion： The first factor affecting extractor productivity was volume percentage of ethonal. The second and the third ones were extracting frequency and time respectively.