灵芝破壁孢子粉改善睡眠作用研究

Studies of Cracked Lingzhi Spores Powder on hypnotic effects in mice

目的观察灵芝破壁孢子粉的改善睡眠作用。方法将清洁级ICR雄性小鼠随机分为对照组及灵芝破壁孢子粉低、中、高剂量组,每组15只。对照组灌胃给予0.5%羧甲基纤维素钠20mL/kg,灵芝破壁孢子粉低、中、高剂量组分别灌胃给予灵芝破壁孢子粉0.25、0.5、1.0mg/kg,每日1次,连续灌胃30天。观察小鼠直接睡眠的入睡动物数及睡眠时间,戊巴比妥钠阈下剂量催眠的睡眠发生率,戊巴比妥钠阈剂量催眠的睡眠时间及巴比妥钠诱导小鼠睡眠潜伏期。结果灵芝破壁孢子可明显延长戊巴比妥钠诱导的小鼠睡眠时间,缩短巴比妥钠诱导的小鼠睡眠潜伏期,提高戊巴比妥钠诱导的小鼠睡眠发生率,对直接睡眠小鼠入睡动物数及睡眠时间均无明显影响。结论灵芝破壁孢子粉具有改善睡眠作用。

Objective To observe the hypnotic effects of Cracked Lingzhi Spores Powder in mice. Methods Healthy ICR mice were randomly divided into control group, and low, middle and high groups of Cracked Lingzhi Spores Powder. Control group was treated by 0.5% CMC-Na 20ml/kg with intragastric daministration, low, middle and high groups of Cracked Lingzhi Spores Powder were treated by Cracked Lingzhi Spores Powder 0.25, 0.5 and 1.0mg/kg with intragastric daministration. One time every day, continuous intragastric daministration for 30 days.The effect of sleep improvement was studied by the expeximents of sleep-induced directly, prolonging sleeping time induced by pentobarbital sodium, affecting mice sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium and affecting sleeping deliteseenee induced by tobarbital sodium. Results Cracked Lingzhi Spores Powder could significantly prolong the time of sleep induced by pentobarbital sodium, shorten sleep latent period induced by barbital sodium, and boost sleep incidence rate induced by subthreshold dose of pentobm ital sodium, but had no significant effects on the direct sleep of mice. Conclusion Cracked Lingzhi Spores Powder could improve the sleep function of mice.