培养条件对平贝母愈伤组织生长和生物碱积累的影响研究

Studies on the effects of culture conditions to growth and alkaloid accumulation of the callus from Fritillaria ussurionsis

目的了解培养条件对平贝母愈伤组织生长和总生物碱含量的影响。方法分别以光暗培养、氮源比例、碳源种类、蔗糖浓度、磷酸盐浓度作为影响因子进行愈伤组织培养。结果光培养条件、NH4+:NO3-=1:1、蔗糖4%、磷酸盐浓度1.5mmol·L-1时,平贝母愈伤组织生长较快、总生物碱积累较多,结论培养条件对平贝母愈伤组织生长和总生物碱积累均有影响。

Objective To understand the effects of cuhure conditions to growth and alkaloid accumulation of the callus from Fritillaria ussurionsis. Methods The conditions of light and dark culture, NH4＋：NO3- portion, different carbohydrates, sucrose concentration and phosphate were used as effective factors for the experiment. Results The condition of light, NH4＋：NO3- portion 1：1, sucrose concentration 4% and phosphate 1.5mmol ·L^-1 is optimal for the callus growth and alkaloid accumulation. Conclusion The culture conditions affect the growth and alkaloid accumulation of the callus from Fritillaria ussurionsis.