丹参酮ⅡA对脑室周围白质软化新生大鼠白细胞介素-1β、白细胞介素-6表达的影响

Effects of Tan II A on expressions of interleukin-1β and interleukin-6 in neonatal rats with periventricular leu- komalacia

目的探讨丹参酮ⅡA（TanHA）对脑室周围白质软化（PVL）新生大鼠脑组织病理及白细胞介素-1β（IL-1β）、IL-6的调节作用。方法2Ft龄新生大鼠结扎双侧颈总动脉并吸入80mL／L氧气和920mL／L氮气混合气体0．5h建立PVL模型，随机分为模型对照组、TanⅡA低剂量组[7．5mg／（kg·d）]及TanⅡA高剂量组[15．0mg／（kg·d）]，设立假手术组，分别于第3天给药后1h处死，HE染色检测脑组织病理变化，双抗体夹心酶联免疫吸附法（ELISA）检测IL-1β和IL-6水平。结果模型对照组侧脑室旁细胞排列紊乱，白质稀疏，呈筛网状坏死，缺血性改变明显；TanⅡA低剂量组较模型对照组缺血明显改善，而TanHA高剂量组较低剂量组缺血改善更明显。PVL新生大鼠IL-1β及IL-6表达水平均明显高于假手术组（P均〈0．01）；TanⅡA高剂量、低剂量均能显著降低IL-1β及IL-6表达水平，与模型对照组比较，差异均有统计学意义（P均〈0．05）；而TanⅡA低剂量组与高剂量组比较，差异均无统计学意义（P均〉0．05）。结论TanⅡA可以显著改善PVL新生大鼠脑组织缺血情况、降低炎性因子IL-1β和IL-6的表达，减轻新生大鼠脑白质损伤。

Objective To study the effects of Tan lI A on expressions of interleukin-1β（IL-1β） and interleu- kin-6 （IL-6） in brain tissues of neonatal rats with periventricular leukomalacia （PVL）. Methods The PVL models were established by means of bilateral general cervical artery ligation followed by 0.5 h 80 mL/L oxygen and 920 mL/L nitrogen mixture exposure in 2-day rats, and then these rats were randomly divided into the model control group, the low- dose group treated with Ta Ⅱ A at 7.5 rag/（ kg ~ d） and the high-dose group treated with TaⅡ A at 15.0 rag/（ kg ~ d）, meanwhile the false operation group was established. The rats were killed 1 hour after administration on the third day, and the brain tissues pathological changes were detected by way of HE dyeing analysis. At the same time, the expres- sions of IL-1β and IL-6 in brain tissues were analyzed by means of enzyme linked immunosorbent assay（ELISA）. Re- suits HE staining showed that the ceils around the lateral ventricle were disarranged,as white matter appeared sparse as cribriform and ischemic changed significantly in the rats of model control group. Compared with the model control group, the ischemia of brain tissue in the low-dose group improved. Ischemia of the high-dose Tan II A group improved more significantly than the low-dose group. ELISA results showed that the expressions of IL-1β and IL-6 in the PVL rats were significantly higher than the false operation group （ all P 〈 0.01 ）, both of which were statistically decreased in the high-dose and the low-dose Tan ⅡA groups （ all P 〈 0.05 ） ; and there was no significant difference between the low- dose group and the high-dose group （ all P 〉 0.05 ）. Conclusions Tan Ⅱ A has the function of improving the state of ischemia and decreasing the expressions of IL-1β and IL-6 in tissues of PVL rats,thus it can relieve cerebral white mat- ter lesions of neonatal rats.