早期心肌梗死bFGF的免疫组化研究

Immunohistochemical study of basic fibroblast growth factor of early myocardial infarction

采用免疫组化和图像分析与统计学处理方法，对人体早期心肌梗死猝死者心肌中碱性成纤维细胞生长因子 (basic fibroblast growth factor,bFGF)的表达进行了研究。结果发现 :心肌梗死组 24例的心肌细胞核以及血管内皮细胞核均为强阳性染色，有些细胞浆也有阳性染色，尤以心肌梗死灶周围区域心肌细胞为甚。可疑梗死组 21例中 17例见心肌细胞核以及血管内皮细胞核为强阳性染色， 4例为弱阳性染色，未见阴性染色。而正常心脏对照组 16例中 3例呈弱阳性染色，其余均为阴性。图像分析与统计学处理结果显示对照组 bFGF阳性指数明显低于心肌梗死组和可疑心肌梗死组（ P< 0.001），而后两组无显著性差异（ P >0.05）。结果表明 bFGF免疫组化染色可望为早期心肌缺血致猝死的死后诊断提供客观的形态学依据。

The expression of basic fibroblast growth factor (bFGF) was first studied in 61 cases of early myocardial infarction. The results revealed that all 24 cases of proven infarcts was positive in nuclei of myocardium and vascular endothelium, and that some was positive in cytoplasm, especially in myocardium around the border zone of infarction. Of 21 suspected infarcts cases, 17 showed strong positive and 4 mild positive. In the control group of 16 samples, only 3 was mild positive sparsely in nuclei of myocardium and vascular endothelium. PI of control group was significantly lower than that of proven and suspected infarcts (P< 0.01). There was no significant difference between PI of proven infarcts and suspected infarcts (P >0.05). These findings suggest that SABC－ bFGF method could be used for morphologic diagnosis of early myocardial infarction of sudden cardiac death.