病毒性心肌炎急性期心肌胶原增生与TGF-β_1的表达

Expression of TGF－β_1 and proliferation of collagen in myocardium in viral myocarditis

目的：研究病毒性心肌炎急性期组织修复性病理改变，探索轻度病毒性心肌炎的法医病理学诊断方法。方法：以适量 Coxsackie B3病毒感染 Balb/c小鼠造成小鼠轻度病毒性心肌炎。利用苦味酸－酸性品红染色法观察病鼠心肌组织胶原纤维的增生情况，并对病鼠及 9例人心肌炎心肌进行 TGF－β 1的 LSAB免疫组化研究。结果：病鼠心肌组织内胶原纤维呈不同程度增生；心肌炎鼠及人心肌炎心肌组织可见大量 TGF－β 1表达； TGF－β 1的表达与胶原纤维的增生呈正相关。结论：病毒性心肌炎急性期即存在组织修复性病理改变， TGF－β 1的阳性表达是反映心肌炎症性损伤修复的灵敏指标。

To investigate the tissue repair of acute viral myocarditis,and to explore the diagnostic method of slight viral myocarditis in forensic pathology. Slight viral myocarditis model was induced in Balb/c murine by CVB3. Collagen proliferation in myocardium of mice with myocarditis was observed by special staining.The hearts of mice and human(9 cases) with myocarditis were studied LSAB－ immunohistochemically with anti－ TGF－β 1 antibody.In the study, the proliferation of collagen was seen in myocardium in acute viral myocarditis. Generous expression of TGF－β 1 was found in the myocardium of mice and human with myocarditis. The quantity of collagen proliferation and expression of TGF－β 1 was positive correlation. It is concluded that the tissue repair exists in acute viral myocarditis and that positive staining of myocardium for TGF－β 1 is a sensitive index of myocardial damage and tissue repair.