摘要
采用自制大鼠右顶叶局灶性脑挫伤模型,进行组织学和 Bax/Bcl- 2的免疫组织化学法研究。结果发现 :伤后 12h~ 24h,挫伤灶周围神经细胞和星形胶质细胞形态学发生改变,白细胞附壁和游出;伤后 4d,挫伤灶周围出现大量的泡沫细胞和核大而深染的星形胶质细胞; 8~ 10d,挫伤灶处形成软化灶; 12~ 14d,挫伤灶愈合变成胶质细胞结节,或者变成囊状,可见大量的星型胶质细胞及新生毛细血管,可见含铁血黄素颗粒沉积。 Bax/Bcl- 2表达水平与伤后经历时间有一定相关性。
To set up the experimental model of focal cerebral contusions in rats,and to study the histology and the expression of Bax and Bcl- 2 after cerebral catusions.It was found that neurons and astrocytes around the wound area were changed morphologically and that polymorphonuclear leucocytes were present at 12- 24h after injury. A lot of foamy cells and astrocytes having a big dark nucleus appeared around the wound area on the 4th day after injury. On the 8- 10th day, liquefaction necrosis was seen in areas of contusions. On the 12- 14th day, the contusion focus healed up and became cystiform or glial node. Meantime, lots of astrocytes, capillary vessel and hemosiderin were observed. The expression of Bax and Bcl- 2 increased after injury. There existed a relationship between increased Bax/Bcl- 2 and different intervals after brain injury.
出处
《法医学杂志》
CAS
CSCD
2000年第4期211-213,共3页
Journal of Forensic Medicine
关键词
脑挫伤
损伤时间
BAX
免疫组织化学
法医鉴定
cerebral contusion, timing of injury, apoptosis, bax, bcl- 2, immunohistochemistry, histology