复合扩增9个STR位点在亲子鉴定中的应用评估

Evaluation of 9 STR Loci in paternity identification

在法医学亲子鉴定中应用复合扩增及四色荧光自动分析技术检测 Profiler plusTM9个 STR位点，一次性获得的信息量大，累计非父排除率达 0.9999。应用于 268例亲子鉴定的结果表明， 9个具有高度多态性 STR基因座的联合检测，能使三联体亲子鉴定的排除结论明确无误。对不排除案例，其 RCP值均可达国际认定标准。对二联体亲子鉴定一般需增加 CofilerTM试剂盒 4个 STR位点检测。

9 STR loci obtained by four－ dye fluorescent labeling technique in paternity identification provides much information at one test and the cumulative chance of exclusion gets up to 0.9999. Our result of 268 paternity test cases shows that there are at least two incompatible loci in all Mother－ Child－ Alleged Father (M－ C－ AF) exclusive cases. To those unexclusive cases, The RCP all reaches international standard. It is suggested that more STR loci be used for accurate test in Child－ Alleged Father(C－ AF) case.