桂林市汉族老年非酒精性脂肪性肝病与脂联素基因启动子区-11377C/G单核苷酸多态性的相关性研究

Nonalcoholic Fatty Liver Disease and SNPs-11377C/G in Promoter Region of Adiponectin Gene:A Correlation Study in Han Elderly Population in Guilin

目的探讨桂林市汉族老年非酒精性脂肪性肝病(NAFLD)与脂联素基因启动子区-11377C/G单核苷酸多态性的相关性。方法选择2012年7—11月在桂林医学院附属医院体检中心诊断为NAFLD者150例为NAFLD组,另选择同期与NAFLD组年龄匹配的体检正常者130例为对照组。测量所有受试者身高、体质量、血压,并计算体质指数(BMI);检测其空腹血糖(FBG)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及尿酸(UA)水平。NAFLD影响因素的分析采用单因素Logistic回归分析和非条件多因素Logistic回归分析。采用聚合酶链式反应-限制性片段长度多态性法(PCR-RFLP法)检测脂联素基因启动子区-11377C/G单核苷酸多态性,比较两组基因型及等位基因频率。结果 (1)NAFLD组BMI、收缩压(SBP)、舒张压(DBP)、FBG、ALT、TG、TC、LDL-C及UA水平均高于对照组(P<0.05),HDL-C水平低于对照组(P<0.01);两组AST水平比较,差异无统计学意义(P>0.05)。(2)单因素Logistic回归分析显示,性别、BMI、高血压、FBG、TG、HDL-C、LDL-C、UA进入回归方程(P<0.05);非条件多因素Logistic回归分析显示,性别、BMI、TG、UA进入回归方程(P<0.05)。(3)两组脂联素基因启动子区-11377C/G基因型和等位基因频率比较,差异均无统计学意义(P>0.05)。NAFLD组和对照组不同基因型受试者BMI、SBP、DBP、FBG、ALT、AST、TG、TC、HDL-C、LDL-C及UA水平比较,差异均无统计学意义(P>0.05)。结论桂林市汉族老年NAFLD与脂联素基因启动子区-11377C/G单核苷酸多态性无明显相关性。

Objective To investigate the association of SNPs - 11377 C/G in proximal promoter region of adiponectin gene with nonalcoholic fatty liver disease （NAFLD） in Han elderly population in Guilin. Methods From July to November in 2012, height, weight, blood pressure （BP） were measured, body mass index （BMI） calculated, fasting blood glucose （FBG）, alanine aminotransferase （ALT）, aspartate amiuotransferase （AST）, triglyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （ HDL - C）, low density lipoprotein cholesterol （ LDL - C ） , uric acid （ UA ） detected in 150 NAFLD patients （NAFLD group） and 130 normal subjects （control group）. NAFLD risk factors were analyzed by univari- ate Logistic regression and non- conditional Logistic regression analysis, and SNPs -11377 C/G in proximal promoter region of adiponectin gene detected by polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP）. Genotypes and allele frequency were compared between 2 groups. Results SBP, DBP, FBG and levels of ALT, TG, TC, LDL - C, UA were higher in NAFLD group than in control group （ P 〈 0. 05 ）, HDL - C lower （ P 〈 0. 01 ） ; No significant difference was not- ed in AST between 2 groups （P 〉0. 05）. By univariate Logistic regression analysis, gender, BMI, hypertension, FBG, TG, HDL - C, LDL - C, and UA entered into the regression equation （ P 〈 O. 05 ） ; By non - conditional Logistic regression analy-sis, gender, BMI, TG, UA entered into the regression equation （ P 〈 O. 05 ）. There was no significant difference in SNPs - 11377 C/G in proximal promoter region of adiponectin gene or allele frequency between 2 groups （P 〉 O. 05 ）. There was no sig- nificant difference in BMI, SBP, DBP, FBG, ALT, AST, TG, TC, HDL-C, LDL-C, and UA in the NAFLD patients or normal subjects with different genotypes （P 〉 00.05）. Conclusion There is no obvious correlation between NAFLD and SNPs - 11377 C/G in proximal promoter region of adiponectin gene in Han elderly population in Guilin.