扶芳藤合剂对兔病态窦房结综合征Cx45、Cx43及Cx40蛋白表达的影响

Effect of Fufang Fufangteng Mixture on CX45,CX43 and CX40 Protein in Rabbit with Sick Sinus Syndrome

目的观察复方扶芳藤液对兔病态窦房结综合征模型心率(HR)及窦房结电生理、及心脏窦房结组织Cx45、Cx43及Cx40蛋白表达的影响,并探讨其作用机制。方法将40只清洁级健康日本大耳白兔采用随机、平行对照的方法,按随机数字表分为空白对照组、模型组、心宝丸组及复方扶芳藤液组(中药组)高、低剂量组,每组各8只。造模成功后空白对照组、模型组分别予生理盐水15mL/d,心宝丸组予心宝丸悬浮液,中药组高、低剂量组分别予复方扶芳藤液连续灌胃14d。取各组兔上下腔静脉之间的窦房结组织,常规切片,免疫组化检测窦房结组织Cx45、Cx43及Cx40蛋白表达。结果大剂量组窦房结组织Cx45、Cx43及Cx40蛋白表达明显高于模型组(P<0.05);小剂量组干预后窦房结组织Cx45、Cx43及Cx40蛋白表达较模型组增高,其中,Cx40较模型明显增高(P<0.05)。结论复方扶芳藤可上调损伤兔窦房结组织Cx45、Cx43及Cx40蛋白的表达,可能是治疗病态窦房结综合征的机制之一。

Objective To observe the effect of Fufangteng Mixture on CX45 ,CX43 and CX40 protein in rabbit with sick sinus syn- drome. Methods Forty Japanese white rabbits were randomly divided into five groups：Control group, model group, Xinbao pill group,high dose herb （Fufangteng Mixture） group and low dose herb group,8 rabbits in each group. Control group and model group received physiological distilled water. Xinbao pill group received Xinbao pill. High dose herb group and low dose herb group received Fufangteng Mixture 3.6 g/（kg · d） and 0.9 g/（kg · d） respectively. After 14 days of continuous garaging,sick sinus syndrome models were established by 20% formaldehyde hydropathic compressed on sinus node area. CX45, CX43 and CX40 protein in sinoatrial node tissue were detected by immunohistochemistry. Results CX45,CX43 and CX40 protein in high dose of herb group was significantly higher than that in model group （P〈0.05）. CX40 protein in low dose of herb group was significantly higher than that in model group （P〈0.05）. Conclusion The Fufangteng Mixture could promote the expression of CX45,CX43 and CX40 in sick sinus.