藜豆种质资源遗传多样性的ISSR分子标记分析

Genetic Diversity Analysis of Velvet Bean Germplasm Resources with ISSR Markers

利用ISSR标记构建了9份藜豆种质的指纹图谱，并对其遗传多样性进行评价。结果表明，从35条引物中筛选出8条重复性好的多态性引物进行PCR扩增，共扩增出64条带，其中多态性条带50条，多态性比例为78．1％。应用其中3条多态性ISSR引物（UBC889、UBC812、UBC825）的12条特异性条带，可以有效区分供试的9份藜豆种质资源。藜豆种质问的遗传相似系数变化范围在0．47～0．97之间。利用UPGMA聚类分析，在相似性系数为0．73处可将9个藜豆种质资源划分为3个类群，其亲缘关系与种质地理来源之间存在一定关联性。

Constructed the fingerprint of 9 velvet bean （Stizolobium cochinchinensis） germ- plasm resources and analyse their genetic diversity with ISSR markers. The results indicated that 8 primers with strong stability and repeatability were screened from 35 primers and carried out PCR amplification. 64 bands were amplified from 9 velvet bean varieties, among which 50 were polymor- phic, and the polymorphic rate was up to 78.1%. Using 12 specific bands in the 3 polymorphic ISSR primers （UBC889,UBC812,UBC825）, the 9 velvet bean germplasm resources could be differentiat- ed. The genetic similarity coefficients of velvet bean germplasm ranged from 0. 47 to 0. 97. Nine vel- vet bean varieties were divided into 3 groups by UPGMA cluster analysis with the similarity coeffi- cient of O. 73. The phylogenetic relationship of velvet beans are partly related to the geological sourc- es of the germplasm.