玉米抗虫新种质的创制

Breeding of New Maize Germplasm with Borer Resistance

收集转外源抗虫基因Cry1C*的HiII玉米材料T1代阳性转化单株花粉,与生产上常用的郑58、248、Ye478、昌7-2等30多份自交系测交和回交,并经200 mg/L的草胺膦喷施、PCR检测、大田鉴定等方法对后代植株进行阳性单株筛选,连续进行5次回交和筛选,最终自交纯合获得含Cry1C*基因抗虫玉米新系32份。

Breeding new borer-resistant varieties was an effective method to prevent pests of maize. The pollens from T1 pos- itive plants of HiII transformed with borer-resistant gene Cry1C＊ through particle gun bombardment were collected to pollinate more than 30 important inbreds such as Zheng58, 248, Ye478, Chang7-2 and so on, and then backcrossed with these in- breds as recurrent parents. Subsequently, the positive plants were obtained by 200 mg / L phosphinothricin screening, PCR validation and field identification. After continuous backcrossing and screening for 5 times, 32 new borer-resistant lines with Cry1C＊ gene were bred by final selfing.