猪DIO3基因组织表达谱及印记分析

Expression and Imprinting Analysis of Porcine DIO3 Gene

采用荧光定量PCR方法分析了猪DIO3基因在组织中的表达状况。结果表明,DIO3基因主要表达于背部脂肪组织,在肝脏、子宫、肾脏、心脏、小肠、肌肉和胃组织中呈中度表达,在脾脏和肺脏中表达量很低。采用外显子687 bp处的A/C突变(A/C 687)检测该基因在猪胚胎组织中的印记状态,DIO3基因在所有组织中均表现为印记。

Real-time RT-PCR was performed to analyze the porcine DIO3 gene tissue expression pattern. The porcine DIO3 gene was expressed predominantly in backfat, mildly in liver, uterus, kidney, heart, small intestine, muscle and stomach, and almost absent in spleen and lung by realtime fluorescence quantitative PCR. A single nucleotide polymorphism in exon（A / C687） was used to investigate imprinting status in porcine embryonic tissues. The results indicate that DIO3 was imprinted in all the tested tissues.