大鼠坐骨神经Wallerian溃变的分子机制研究

The study of Wallerian degeneration after rat sciatic nerve injury

目的:运用基因芯片和蛋白芯片技术及生物信息学分析,系统分析大鼠坐骨神经损伤后远端神经组织Wallerian溃变的差异表达基因和蛋白,从基因和蛋白水平探讨Wallerian溃变的分子机制。方法:建立大鼠损伤坐骨神经远端Wallerian溃变模型,利用表达谱基因芯片和抗体蛋白芯片,进行表达趋势分析、功能分析、京都基因与基因组百科全书信号通路分析。通过生物信息学方法全面系统分析,大鼠坐骨神经损伤后远端Wallerian溃变的基因和蛋白表达变化。结果:在Wallerian溃变和再生过程中,共有6076个基因差异表达和23种表达趋势,有93个蛋白差异表达,108种信号通路参与形成Wallerian溃变的信号调控网络。结论:大鼠坐骨神经损伤后Wallerian溃变过程中,有大量基因的表达变化和多种关键因子的调控,本研究为进一步阐明Wallerian溃变的分子机制提供了基本数据,为经典的Wallerian溃变学说增添了新的内容。

Objective： To study the gene expression pattern and signal pathways of the distal nerve stump expressed in Wallerian degeneration （WD） after rat sciatic nerve injury. Methods： WD models of rat sciatic nerve injury were con- structed. A large number of genes involved in WD with the Kyoto Encyclopedia of Genes and Genomes pathway （KEGG） were classified. Signal flow and recurrent neural networks were analyzed by using gene chip microarrays, antibody arrays and bioinformatic analysis. Results： There were 6076 differential genes, 93 differential proteins expressed and 23 types of expression tendencies had significant matches in the database and were assigned to 108 KEGG pathways during Wallerian degeneration. Conclusion： A large number of genes and proteins are differentially regulated during the process of WD. The study will help to much better understand the information for the WD and investigations on the molecular mechanisms of WD regulating nerve degeneration and/or regeneration.