野生粗毛淫羊藿生长周期的形态特征及淫羊藿苷的含量分析

Morphological character of growth cycle for Epimedium acuminatim and icariin content analysis

目的：阐明野生粗毛淫羊藿植物生长周期的形态特征与次生代谢物积累水平。方法：明确生境，运用形态观察、标本制作、生物绘图记录不同时期植物的形态特征；采用HPLC检测不同生长阶段植株各部位的淫羊藿苷含量。结果：粗毛淫羊藿生长周期可划分为营养生长阶段和有性与无性繁殖并存阶段。在林缘旷地生境中，植株需经7～8年的营养生长方开花结实，但在良好营养条件下，生长周期缩短至3～4年。植株营养生长期与有性繁殖期的淫羊藿苷总体水平没有显著性差异。结论：营养生长和有性繁殖初期可通过叶部的形态变化判断生长年限，但植株成年、根茎兼行无性繁殖策略后，难以从形态上判断植株年龄。在药材采挖上，药材品质受生长阶段的影响不明显，只要产量（生物量）达到经济合算，各生长阶段均可采挖。

Objective： To study the relationship between morphological characteristics of growth cycle for Epimedium acuminatium and accumulation level of secondary metabolites. Method： After making habitat clear, methods of morphology observation, specimen preparation, organism charting were applied to record morphological characteristics of E. acuminatium at different stages. HPLC was employed to detect icariin content of different parts of the plant at the stage of vegetative growth and sexual reproduction. Result ： The growth cycle of E. acuminatium was divided into vegetative growth stage and sexual and asexual reproduction coexistence stage. Seven to eight years were needed for the plants flowering and seeding of E. acuminatimcan in open land of forest edge, but within good nutrition conditions, growth cycle is shortened into three to four years. The difference of icariin content in two growth stages was not significant. Conclusion： Morphological change of lower and foliar organ can be utilized to estimate growth age. But after applying sexual propagation for both root and stem of grown plants, it＇s been difficult to determine their growth year. If individual biomass or yield is economical, plants can be harvested in both vegetative growth and sexual reproduction stages.