清热降压胶囊干预SHR海马区组织代谢组学研究

Intervention effects of Qingre jiangya capsule on brain hippocampus of spontaneously hypertensive rats based on metabonomic research

选取30只SHR大鼠随机分为3组模型组,卡托普利组,清热降压胶囊组,wistar大鼠10只作为正常对照组,连续给药14 d后取海马组织,采用核磁共振(1H-NMR)代谢组学方法探讨高血压大鼠的海马组织损伤以及清热降压胶囊的保护作用。研究结果发现卡托普利组海马区代谢轮廓明显异于其余3组,另3组大鼠样本分类的趋势非常明显,提示清热降压胶囊能显著改善大鼠海马组织的代谢,鉴定4种代谢产物为二甲基甘氨酸、甘油磷酸胆碱、醛固酮和去甲肾上腺素。高血压大鼠机体代谢异常主要表现在酪氨酸代谢、血管平滑肌收缩以及醛固酮控制的钠盐再吸收失常,清热降压胶囊在有效降压的同时通过影响这些代谢产物来改善高血压的海马区损伤。

Thirty SHRs were obtained randomly to hypertension, model group, captoprit group and Qingre jiangya capsule group. Ten Wistar rats were used as control group. The hippocampus tissue was removed to explore the damage of spontaneously hypertensive rats（SHR） and the protective effect of Qingre jiangya capsule after continuously administered for 14 days. And then the data were processed by principal component analysis （PCA） and partial least squares discilminant analysis （PLS-DA）. The research results revealed captopill group was significantly different from the other three groups. The classification of other three groups is also very clear after captopril group removed. This suggested that Qingre jiangya capsule could improve the overall metabolism compared with captopill. Four metabolites were identified： dimethylglycine, glycerophosphocholine, aldosterone and noradrenaline. Hypertension hippocampus damage may mainly be expressed in tyrosine metabolism, aldosterone-regulated sodium, vascular smooth muscle contraction reabsorption, and Qingre jiangya capsule could reverse the hippocampus tissue damage of SHR.