宁心痛颗粒干预兔动脉粥样硬化易损斑块效果观察

Effects Observation of the Intervention of Ningxintong Granules for Atherosclerosis Vulnerable Plague of Rabbits

目的:观察宁心痛颗粒对模型兔动脉粥样硬化易损斑块的稳定作用;方法:将50只大白兔均分为5组,分别是A、B1、B2、B3和B4组。A组10只大白兔采用普通饲料喂养,两周后采用球蘘损伤腹主动脉,对球囊损伤部分进行p53基因转染,另外4组大白兔采用高脂饲料喂养,同样在两周后进行球囊损伤腹主动脉,在第10周结束之后,对球囊损伤部分进行p53基因的转染,在12周末将其处死,在将其处死之前的24 h、48 h分别给予两次药物(中国斑点蛙蛇毒和组胺)建立兔动脉粥样硬化易损斑块模型。第3周起B1、B2、B3组分别予宁心痛颗粒高剂量,常规剂量,血脂康胶囊干预,将B4组作为本次研究的模型组,因此不对其进行药物干预。每组大白兔在实验以及处死之前均对其空腹状态下进行血样检测,检测血样中总胆固醇、三酰甘油、低密度脂蛋白以及高密度脂蛋白胆固醇的含量。并对相关的数值进行计算。结果:进行高脂饲料喂养的四组大白兔在喂养12周以后,其血样检测中的四项指标检测结果均明显高于进行普通饲料喂养的A组,且有显著差异(P<0.05),具有统计学意义。进行高脂饲料喂养的B1、B3组大白兔的四项指标检测结果均低于本次研究的模型组B4,且有显著差异(P<0.05),具有统计学意义。但B1、B3组大白兔的四项指标检测水平相当,不具有统计学意义(P>0.05)。B1组的总胆固醇检测结果要明显低于B2组的检测结果,两者结果比较有显著差异(P<0.05),具有统计学意义。其中进行高脂饲料喂养和宁心痛颗粒等干预的三组大白兔的低密度脂蛋白胆固醇、总胆固醇、三酰甘油三者分别和高密度脂蛋白胆固醇的比值,和模型组大白兔的各项比值进行比较,无显著差异(P>0.05),不具有统计学意义。结论:宁心痛颗粒可以对炎症有一定的抑制作用,进而稳定干预模型兔动脉粥样硬化易损斑块。

Objective：To observe the stabilizing effects of Ningxintong Granule on the atherosclerosis vulnerable plague of rabbits. Methods ：50 rabbits were randomly divided into group A （6 rats） , group B1 （9 rats）, group 132 （9 rats ）, group B3 （9 rats） and group 134（9 rats）. Group A were given common feed for two weeks, amt then balloon injured method and exogenous p53 gene transfection was applied. Group BI, B2, B3 andlM were fed with high fat diet for 2 weeks, and then the Abdominal aorta balloon injury was applied. In the end of the 10th week,the balloon injury area was applied p53 gene transfeetion. In the end of the 12th week,the rabbits were executed. 48h and 24h before the execution, the rabbits were administrated with drugs （Chinese speckled frog snake venom and histamine） in order to establish the model of atherosclerosis vulnerable plague of rabbits. From the 3rd week,high dosage and ruutine dosage of Ningxintong Granules and Xuezhikang Capsule were given to group BI ,group B2,group B3 respectively while group B4 without drug intervention were observed as the model group. The TCH, TG, HDL - C, LDL - C of each group before the experiment as well as after their execution were detected by lasting blood test. Results：After 12 weeks of high fat feed,the levels of TCH,TG,LDL- C and HDL- C in groups BI ,B2,B3 and B4 were significantly improved when com- pared with that of group A. The difference had statistical significance（ P 〈 0.05） ;the levels of TCH, TG and LDL -C in group B1 were lower than those of group B4. The difference had statistical significance （P 〈 0.05 ） ;the levels of TCH, LDL- C and HDL- C in group B3 were lower than those of group B4. The difference had statistical significance（ P 〈 0.05 ） ;the ratios of LDL -C/ HDL - C ,TG/HDL - C and TCH/HDL - C in group BI, B2, B3 were not significantly different from those of gnmp tM （ P 〉 0. 05 ） ;the levels of TCH,TG,LDL - C and HDL - C in group BI was similar to those of group B3 and the difference had no statisti- cal significance（ P 〉 O. 05 ） ;the level of TCH in group B1 was lower than that of group B2 and the difference had statistical signif- icance（ P 〈 0.05 ）. Conc]usion：Ningxintong Granule has stabilizing effects on the atberoscierosis vulnerable plague of rabbits.