补中益气丸补气疗效的谱-效关系研究

Spectrum-effect Relationship of Tonifying Qi Efficacy of Buzhong Yiqi Pills

目的:探讨补中益气丸高效液相色谱(HPLC)指纹图谱与其补气作用的关联性,确立其补气药效成分。方法:建立补中益气丸的HPLC-二极管阵列检测器(DAD)和HPLC-蒸发光散射检测器(ELSD)指纹图谱;用控食法和疲劳法联合复制小鼠气虚模型,测定小鼠体质量、脏器指数、廓清指数和矫正廓清指数;用灰关联度分析方法研究其谱-效关系。结果:HPLC-DAD指纹图谱中各峰显示橙皮苷(峰号11)、芒柄花素(峰号19)对补气作用有较大贡献。HPLC-ELSD指纹图谱中各峰显示橙皮苷(峰号4)对补气作用有较大贡献。结论:补中益气丸对模型小鼠气虚症状有明显改善作用;HPLC-DAD和HPLC-ELSD指纹图谱均与补中益气丸的补气作用有一定对应关系;补中益气丸中对补气作用贡献较大的成分是橙皮苷、芒柄花素。

OBJECTIVE： To analyze the correlation between HPLC fingerprints of Buzhong yiqi pills and tonifying qi efficacy, and to establish its medicinal components group of tonifying qi efficacy. METHODS： HPLC-DAD and HPLC-ELSD fingerprint of Buzhong yiqi pills were established; qi deficiency mice model was induced by controlling diet and fatigue test; body weight, viscera index, clearance index and correct clearance index were determined. The spectrum-effect relationship was studied using gray relational grade. RESULTS： The tonifying qi efficacy of each peak in HPLC-DAD fingerprint were hesperidin （peak 11） and formononetin（peak 19）. The tonifying qi efficacy of each peak in HPLC-ELSD fingerprint was hesperidin（peak 4）. CONCLUSIONS： Buzhong yiqi pills have obvious improvement for qi deficiency symptoms of mice. There is certain corresponding relation between HPLC-DAD, HPLC-ELSD fingerprint and tonifying qi efficacy of Buzhong yiqi pills. Hesperidin, formononetin in Buzhong Yiqi pills is big role in buqi effection.