GC法测定不同来源苍术饮片中茅术醇和β-桉叶醇的含量

Content Determination of Hinesol and β-eudesmol in Atractylodis Rhizoma Decoction Pieces from Different Sources by GC

目的：建立测定苍术饮片中茅术醇和伊桉叶醇含量的方法，并对不同来源苍术饮片中二者含量进行测定。方法：以正十六醇为内标物，采用气相色谱法进行测定。色谱柱为HP-5石英弹性毛细管柱（30m×0．32min×0．25μm），载气为氮气，检测器为氢火焰检测器（FID），采用程序升温方式，分流进样（10：1），进样口温度为220℃，检测器温度为250℃。结果：茅术醇和口．桉叶醇的进样量分别在0．40～2．40、0．08～0．48gg范围内同内标物的比值与各自峰面积积分值呈良好的线性关系（r分别为0．9998、0．9991）；精密度、稳定性、重复性试验的RSD〈3％；平均加样回收率分别为99．15％、101．04％，RSD分别为1．51％、1．52％（n均为6）。不同来源苍术饮片中茅术醇和伊桉叶醇的含量差异较大，以湖北英山药材中二者含量最高。结论：该方法简便、准确、重复性好，可用于苍术饮片的质量控制。

OBJECTIVE： To establish the method for the content determination of hinesol and fl-eudesmol in Atractylodis Rhizoma decoction pieces, and to determine the contents of them in samples from different sources. METHODS： GC was applied with n-hexadecanol as the internal standard. HP-5 quartz elasticity capillary column （30 m×0.32 mm×0.25 μm）, nitrogen as carrier gas, FID detector, temperature programming, split sampling（ 10 ： 1 ）, injector temperature of 220 ℃, detector temperature of 250 ℃. RESULTS： The linear range was 0.40-2.40 μg for hinesol（r=0.999 8） and 0.08-0.48 μg for β-eudesmol （r=0.999 1）. The average recovery rate of hinesol was 99.15% （RSD= 1.51%, n=6） and that of β-eudesmol was 101.04% （RSD= 1.52%, n=6）. RSDs of precision, stability and reproducibility tests were 〈3%. The contents of hinesol and β-eudesmol from different sources were significantly different from each other, among which those of samples from Hubei Yingshan was the highest. CONCLUSIONS： The method is simple, accurate and reproducible, and suitable for the quality control of Atractylodis Rhizoma decoction pieces.